5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

Background No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. Aim To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Methods Twenty-six Wistar rats were divided into...

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Bibliographic Details
Published in:Digestive diseases and sciences 2011-04, Vol.56 (4), p.1037-1046
Main Authors: Caltabiano, Caroline, Máximo, Felipe Rodrigues, Spadari, Ana Paula Pimentel, da Conceição Miranda, Daniel Duarte, Serra, Marcia Milena Pivatto, Ribeiro, Marcelo Lima, Martinez, Carlos Augusto Real
Format: Article
Language:English
Subjects:
Analysis
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Biochemistry
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Colitis - drug therapy
Colitis - pathology
Colon - drug effects
Colon - pathology
DNA
DNA damage
DNA Damage - drug effects
Feces
Feeding. Feeding behavior
Free radicals (Chemistry)
Fundamental and applied biological sciences. Psychology
Gastroenterology
Genetic research
Hepatology
Hydrogen Peroxide - adverse effects
Intestinal Mucosa - drug effects
Intestinal Mucosa - pathology
Male
Medical sciences
Medicine
Medicine & Public Health
Mesalamine
Mesalamine - pharmacology
Oncology
Original Article
Oxidative Stress - drug effects
Pharmacology. Drug treatments
Rats
Rats, Wistar
Transplant Surgery
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Background No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. Aim To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Methods Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4 weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H 2 O 2 challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level ( P  
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-010-1378-z