2-Aminobenzoic acid of Bacillus sp. BS107 as an ISR determinant against Pectobacterium carotovorum subsp. carotovotrum SCC1 in tobacco

Root drench-treatment of tobacco seedlings with Bacillus sp. BS107 (BS107) suppressed disease development caused by the pathogen Pectobacterium carotovorum subsp. carotovotrum SCC1 (SCC1). A determinant of BS107 involved in induced systemic resistance (ISR) against SCC1 was isolated from cell-free c...

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Bibliographic Details
Published in:European journal of plant pathology 2011-03, Vol.129 (3), p.371-378
Main Authors: Yang, Si Young, Park, Myung Ryeol, Kim, In Seon, Kim, Young Cheol, Yang, Jung Wook, Ryu, Choong-Min
Format: Article
Language:English
Subjects:
Agriculture
Aminobenzoic acid
Bacillus
Bacillus sp. BS107
Bacteria
Bacterial plant pathogens
Bioassays
Biological and medical sciences
Biomedical and Life Sciences
Ecology
Fundamental and applied biological sciences. Psychology
Gene expression
Induced systemic resistance
Life Sciences
Mass spectrometry
Metabolites
Pathogens
Pectobacterium
Phytopathology. Animal pests. Plant and forest protection
Plant diseases
Plant Pathology
Plant resistance
Plant Sciences
Seedlings
Soft-rot disease
Tobacco
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Summary:Root drench-treatment of tobacco seedlings with Bacillus sp. BS107 (BS107) suppressed disease development caused by the pathogen Pectobacterium carotovorum subsp. carotovotrum SCC1 (SCC1). A determinant of BS107 involved in induced systemic resistance (ISR) against SCC1 was isolated from cell-free culture. ISR bioassay-guided isolation was involved in determining active fractions during chromatography. Mass spectrometry and NMR analyses of the isolated metabolite identified 2-aminobenzoic acid (2-AB) as a main ISR determinant. 2-AB at 2.3 mM suppressed significantly disease development and exhibited no direct contact inhibition of pathogen. Reverse Transcriptase (RT)-PCR analyses of tobacco leaves revealed up-regulation of the induced resistance marker genes such as PR1a, PR1c, PR2 and PR4 by application of 2-AB on the root. Among aminobenzoic acids tested, 2- and 4-aminobenzoic acids showed ISR activity against soft-rot pathogen, but 3-aminobenzoic acid did not. This is the first report that 2-AB exhibits the ISR against SCC1in tobacco. BS107 can play a role in promoting plant defences by secretion of bacterial determinants including 2-AB for elicitation of ISR.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-010-9687-9