Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler...

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Bibliographic Details
Published in:African journal of biotechnology 2010-07, Vol.9 (30), p.4644-4650
Main Authors: Cingel, A, Vinterhalter, B, Vinterhalter, D, Calic-Dragosavac, D, Smigocki, A, Ninkovic, S
Format: Article
Language:English
Subjects:
Solanum tuberosum
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Summary:An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.
ISSN:1684-5315
1684-5315