Antioxidant and hepatoprotective activities of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves

• Published in: Food and chemical toxicology 2011-09, Vol.49 (9), p.2422-2428
• Main Authors: Maheshwari, D.T., Yogendra Kumar, M.S., Verma, Saroj K., Singh, Vijay K., Singh, Som Nath
• Format: Article
• Language: English
• Subjects: alanine transaminase; Animals; antioxidant activity; Antioxidant enzymes; antioxidants; Antioxidants - pharmacology; aspartate transaminase; bilirubin; Biological and medical sciences; blood serum; body weight; carbon tetrachloride; Carbon Tetrachloride - toxicity; catalase; Chemical and Drug Induced Liver Injury - prevention & control; gallic acid; glutathione; glutathione peroxidase; glutathione transferase; glutathione-disulfide reductase; Hippophae - chemistry; Hippophae rhamnoides; histopathology; hydroperoxides; isorhamnetin; kaempferol; leaves; Lipid peroxidation; liver; Liver - drug effects; Medical sciences; myricetin; necrosis; oral administration; oxidative stress; Phenolic compounds; Phenols - pharmacology; Plant Leaves - chemistry; Protein oxidation; quercetin; Rats; Rats, Sprague-Dawley; reversed-phase high performance liquid chromatography; Seabuckthorn; superoxide dismutase; Total phenol; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2011.06.061

► Preparation of phenolic rich fraction from Seabuckthorn leaves. ► Evaluation of antioxidant and hepatoprotective activities of phenolic rich fraction. ► A simple RP-HPLC method for the quantitative analysis of five phenolic compounds. Present study was aimed to investigate antioxidant and hepatoprotective activities of phenolic rich fraction (PRF) of Seabuckthorn leaves on CCl4 induced oxidative stress in Sprague Dawley rats. Total phenolic content was found to be 319.33mg gallic acid equivalent (GAE)/g PRF and some of its phenolic constituents, such as gallic acid, myricetin, quercetin, kaempferol and isorhamnetin were found to be in the range of 1.935–196.89mg/g of PRF as determined by reverse-phase high-performance liquid chromatography (RP-HPLC). Oral administration of PRF at dose of 25–75mg/kg body weight significantly protected from CCl4 induced elevation in aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transpeptidase (GGT) and bilirubin in serum, elevation in hepatic lipid peroxidation, hydroperoxides, protein carbonyls, depletion of hepatic reduced glutathione (GSH) and decrease in the activities of hepatic antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione-S-transferase (GST). The PRF also protected against histopathological changes produced by CCl4 such as hepatocytic necrosis, fatty changes, vacuolation, etc. The data obtained in the present study suggests that PRF has potent antioxidant activity, prevent oxidative damage to major biomolecules and afford significant protection against CCl4 induced oxidative damage in the liver.