Variability and phylogenetic relationships of the Cucumis sativus L. species inferred from NBS-profiling and RAPD analysis

• Published in: Russian journal of genetics 2011-08, Vol.47 (8), p.931-941
• Main Authors: Goryunova, S. V., Gashkova, I. V., Kosareva, G. A.
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; Cucumis; Cucumis sativus; Human Genetics; Microbial Genetics and Genomics; Plant Genetics
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795411080060

Genetic variability of the Cucumis sativus species and its phylogenetic relationsips with other species of the genus were studied on the basis of RAPD marking and analysis of intra- and interspecific polymorphism of the nucleotide sequences of the NBS-LRR gene family in species of the genus Cucumis with the use of the NBS-profiling method. According to RAPD analysis, cucumber cultivars from different geographic regions are highly similar, except for accessions k-3835 and k-3833 from Afghanistan. NBS-profiling analysis revealed phylogenetically most distinct accessions expected to be characterized by specificity of resistance: k-3845 from Uzbekistan, k-3851 from Kyrgyzstan, line 701, k-3835 and k-3833 from Afghanistan, k-2757 and k-3079 from Netherlands, vr.k. 908 from Canada, k-2926 from Bulgaria, Russian cultivars Monastyrskii, Izyashchnyi, and Lel’. Three essentially different groups of species were distinguished, and the C. sativus species (subgenus Cucumis ) was found to be distant from the species belonging to the subgenus Melo .