Molecular approaches to analysing the microbial composition of raw milk and raw milk cheese

The availability and application of culture-independent tools that enable a detailed investigation of the microbiota and microbial biodiversity of food systems has had a major impact on food microbiology. This review focuses on the application of DNA-based technologies, such as denaturing gradient g...

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Bibliographic Details
Published in:International journal of food microbiology 2011-11, Vol.150 (2), p.81-94
Main Authors: Quigley, Lisa, O'Sullivan, Orla, Beresford, Tom P., Ross, R. Paul, Fitzgerald, Gerald F., Cotter, Paul D.
Format: Article
Language:English
Subjects:
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Typing Techniques - methods
Biodiversity
Cheese - microbiology
Colony Count, Microbial
Culture-independent microbiology
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Food Microbiology
Humans
Microbial composition
Milk - chemistry
Milk - microbiology
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Raw milk/cheese
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Summary:The availability and application of culture-independent tools that enable a detailed investigation of the microbiota and microbial biodiversity of food systems has had a major impact on food microbiology. This review focuses on the application of DNA-based technologies, such as denaturing gradient gel electrophoresis (DGGE), temporal temperature gradient gel electrophoresis (TTGE), single stranded conformation polymorphisms (SSCP), the polymerase chain reaction (PCR) and others, to investigate the diversity, dynamics and identity of microbes in dairy products from raw milk. Here, we will highlight the benefits associated with culture-independent methods which include enhanced sensitivity, rapidity and the detection of microorganisms not previously associated with such products. ► Demonstrates the benefits of employing molecular biology for dairy microbiology investigations. ► Summarises a vast quantity of papers which have unveiled the rich microbial diversity of raw milk and raw milk cheeses. ► Reveals the benefit of culture independent approaches when investigating the microbial quality/safety of these products.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2011.08.001