Enhanced chondrogenic marker expression of human mesenchymal stem cells by interaction with both TGF- beta 3 and hyaluronic acid

This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF- beta 3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes wit...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and applied biochemistry 2011-08, Vol.58 (4), p.271-276
Main Authors: Bhang, SH, Jeon, J-Y, La, W-G, Seong, J Y, Hwang, J W, Ryu, SE, Kim, B-S
Format: Article
Language:English
Subjects:
Cell culture
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF- beta 3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes with or without TGF- beta 3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage-specific markers using real-time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA-coated dishes with TGF- beta 3 supplementation revealed a prominent increase in collagen type II, aggrecan, and SoX9. When hMSCs were cultured without TGF- beta 3 supplementation, only hMSCs cultured on HA-coated dishes showed prominent expression of the cartilage-specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell-adhesion matrix and a soluble growth factor.
ISSN:0885-4513
DOI:10.1002/bab.39