Cadherin-integrated liposomes with potential application in a drug delivery system

Abstract N-cadherin (CDH2) proteins were reconstituted with liposomes using a baculovirus expression-liposome fusion method. CDH2 budded viruses were fused with giant liposomes containing dioleoylphophogycerol/dioleoylphosphatidylcholine (DOPG/DOPC) at pH 4.5 and the localization of CDH2 on the lipo...

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Bibliographic Details
Published in:Biomaterials 2011-12, Vol.32 (36), p.9899-9907
Main Authors: Kamiya, Koki, Tsumoto, Kanta, Yoshimura, Tetsuro, Akiyoshi, Kazunari
Format: Article
Language:English
Subjects:
Adhesiveness
Advanced Basic Science
Baculovirus
Biomedical materials
Blotting, Western
Cadherin
Cadherins - metabolism
Cell Communication
Cell Line, Tumor
Confocal
Dentistry
Drug Delivery Systems - methods
Endosomes
Endosomes - metabolism
Human
Humans
Lasers
Liposome
Liposomes
Liposomes - metabolism
Liposomes - ultrastructure
Lysosomes - metabolism
Membrane protein
Models, Biological
Proteoliposome
Recombinant Proteins - metabolism
Viruses
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Summary:Abstract N-cadherin (CDH2) proteins were reconstituted with liposomes using a baculovirus expression-liposome fusion method. CDH2 budded viruses were fused with giant liposomes containing dioleoylphophogycerol/dioleoylphosphatidylcholine (DOPG/DOPC) at pH 4.5 and the localization of CDH2 on the liposome membrane was observed by confocal laser scanning microscopy. CDH2 liposomes showed Ca2+ -dependent association. CDH2-mediated association/dissociation in CDH2 liposomes was specific to Ca2+ and reversible. CDH2-expressing LN-229 cells (human glioblastoma cell) adhered to CDH2 liposomes and small CDH2 liposomes (diameter approximately 150 nm), in particular, were internalized by endocytosis and partly escaped endosomes. Cadherin-containing liposomes show high potential as a new cell-specific proteoliposome. The baculovirus expression-liposome fusion method is useful as a new enabling technology for biomedical applications of functional proteoliposomes.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.09.008