In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua

Polygonatum cyrtonema Hua. lectins (PCLs) were extracted from plantlets regenerated from rhizome explants of P. cyrtonema. Rhizome explants demonstrated a high frequency of callus induction (72.5%) and adventitious shoots differentiation (83.7%) on Murashige Skoog (MS) medium supplemented with 2.0 m...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2009-12, Vol.99 (3), p.269-275
Main Authors: Zhao, Qi, Wu, Chuanfang, Wang, Wenguo, Yuan, Shu, Bao, Jinku, Chen, Fang
Format: Article
Language:English
Subjects:
adventitious shoots
Antibodies
Aquatic plants
Benzyladenine
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Blood cells
Callus
callus formation
cell differentiation
culture media
Dichlorophenoxyacetic acid
Explants
Fundamental and applied biological sciences. Psychology
Gene expression
genes
hemagglutination
in vitro regeneration
Lectins
Life Sciences
Mannose
Mannose-binding lectin
medicinal plants
messenger RNA
mortality
Naphthaleneacetic acid
Original Paper
Plant extracts
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plantlets
Polygonatum
Polygonatum cyrtonema
Polymerase chain reaction
Regeneration
reverse transcriptase polymerase chain reaction
Rhizomes
rooting
Shoots
Survival
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Summary:Polygonatum cyrtonema Hua. lectins (PCLs) were extracted from plantlets regenerated from rhizome explants of P. cyrtonema. Rhizome explants demonstrated a high frequency of callus induction (72.5%) and adventitious shoots differentiation (83.7%) on Murashige Skoog (MS) medium supplemented with 2.0 mg l⁻¹ 2,4-dichlorophenoxyacetic acid and 1.0 mg l⁻¹ 6-benzyladenine. The adventitious shoots could root readily on 1/2 MS medium + 0.5 mg l⁻¹ α-naphthaleneacetic acid and regenerate plantlets with a survival rate of 75.0%. Regenerated rhizomes were freeze-dried, macerated and prepared for total RNAs and proteins extraction. The PCL gene was cloned and its expression level was measured by RT-PCR. Western blot using a lectin-specific antibody revealed a similar amount in regenerated rhizomes compared to wild rhizomes, Furthermore, lectin derived from regenerated rhizomes retained its ability to haemagglutinate rabbit blood cells.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-009-9600-4