New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH

Chromosome painting (CP) with a probe of B chromosome obtained by microdissection and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, C o t−1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but not rDNA between A and B chromosomes in the fish Astyanax s...

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Bibliographic Details
Published in:Genetica 2011-08, Vol.139 (8), p.1073-1081
Main Authors: Vicari, Marcelo Ricardo, de Mello Pistune, Helena Flávia, Castro, Jonathan Pena, de Almeida, Mara Cristina, Bertollo, Luiz Antonio Carlos, Moreira-Filho, Orlando, Camacho, Juan Pedro M, Artoni, Roberto Ferreira
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Astyanax scabripinnis
Biomedical and Life Sciences
Characidae - genetics
Chromosome Painting
Chromosomes
Chromosomes - genetics
cytogenetic analysis
DNA - genetics
Evolutionary Biology
Female
fish
fluorescence in situ hybridization
Human Genetics
Life Sciences
Male
Meiosis - genetics
Metaphase - genetics
Microbial Genetics and Genomics
Microdissection
Plant Genetics and Genomics
Probes
Repetitive Sequences, Nucleic Acid - genetics
ribosomal DNA
satellite DNA
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Summary:Chromosome painting (CP) with a probe of B chromosome obtained by microdissection and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, C o t−1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but not rDNA between A and B chromosomes in the fish Astyanax scabripinnis. Meiotic analysis revealed a pachytene B chromosome bivalent nearly half the size of its mitotic configuration, suggesting a self-pairing of B chromosome arms. Such an isochromosome nature of somatic B chromosome was further evidenced by CP and FISH. All the findings obtained suggest (i) intraspecific origin of B chromosome, and (ii) evolutionary enrichment of repetitive DNA classes, especially those contained in the C o t−1 and the As51 probes, in B chromosome. However, the precise origin of B chromosome in the present species remains to be elucidated by further molecular cytogenetic analysis because of painting of some A chromosome regions with the B chromosome-derived probe.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-011-9611-z