Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants

Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm' region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic di...

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Bibliographic Details
Published in:Russian journal of genetics 2010, Vol.46 (1), p.36-42
Main Authors: Boronnikova, S. V, Kalendar, R. N
Format: Article
Language:English
Subjects:
Adonis vernalis
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedicine
Human Genetics
Microbial Genetics and Genomics
Plant Genetics
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Summary:Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm' region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity of A. vernalis populations were determined.
ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795410010060