Characterization of a novel β-lactamase-producing plasmid in Neisseria gonorrhoeae: sequence analysis and molecular typing of host gonococci

Objectives To determine the complete nucleotide sequence of the novel Johannesburg β-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing. Methods Eleven PPNG i...

Full description

Saved in:
Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2011-07, Vol.66 (7), p.1514-1517
Main Authors: Müller, Etienne E., Fayemiwo, Samuel A., Lewis, David A.
Format: Article
Language:English
Subjects:
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial diseases
Bacterial diseases of the genital system
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Biological and medical sciences
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Epidemiology. Vaccinations
Female
General aspects
Genotype
Gonorrhea - microbiology
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Molecular Sequence Data
Molecular Typing
Neisseria gonorrhoeae
Neisseria gonorrhoeae - classification
Neisseria gonorrhoeae - enzymology
Neisseria gonorrhoeae - genetics
Neisseria gonorrhoeae - isolation & purification
Pharmacology. Drug treatments
Sequence Analysis, DNA
Sequence Deletion
South Africa
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives To determine the complete nucleotide sequence of the novel Johannesburg β-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing. Methods Eleven PPNG isolates containing the Johannesburg β-lactamase-encoding plasmid were previously identified among gonococci isolated from men with urethral discharge attending a clinic in Alexandra (Johannesburg) using a PCR assay. DNA sequence-based characterization of one such plasmid was performed to determine its relatedness to the prototype Asia plasmid. The 11 PPNG isolates containing the Johannesburg plasmid and 105 other clinical gonococci isolates were typed using N. gonorrhoeae multi-antigen sequence typing (NG-MAST). Results Plasmid pEM1 was determined to comprise 4865 bp and to be a deletion derivative of the prototype Asia plasmid with a unique 2560 bp deletion in the non-TnA region. NG-MAST genotyping demonstrated a significant association between sequence type (ST) 502, or other closely related STs, and the Johannesburg plasmid-containing PPNG (P 
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkr162