Probing of some compunds as anti-aggregatory additives in the protein refolding process from Escherichia coli inclusion bodies

Five compounds of different chemical structure were tested for aggregation suppression during the refolding of porcine and mink growth hormones as model proteins from Escherichia coli inclusion bodies by the dilution method. Of all compounds tested in this work, 3-guanidinopropionic acid (GPA) conta...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2011-08, Vol.58 (4), p.277-284
Main Authors: Zilinskas, A, Sereikaite, J
Format: Article
Language:English
Subjects:
Arginine
Escherichia coli
Online Access:Get full text
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Summary:Five compounds of different chemical structure were tested for aggregation suppression during the refolding of porcine and mink growth hormones as model proteins from Escherichia coli inclusion bodies by the dilution method. Of all compounds tested in this work, 3-guanidinopropionic acid (GPA) containing a guanidinium group was the most effective additive for aggregation suppression. Anti-aggregatory properties of GPA were compared with the ones of L-arginine.
ISSN:0885-4513
DOI:10.1002/bab.40