Characterization of p53 expression in rainbow trout

The tumour suppressor protein p53 is a critical component of cell cycle checkpoint responses. It upregulates the expression of cyclin-dependent kinase inhibitors in response to DNA damage and other cellular perturbations, and promotes apoptosis when DNA repair pathways are overwhelmed. Given the hig...

Full description

Saved in:
Bibliographic Details
Published in:Comparative biochemistry and physiology. Toxicology & pharmacology 2011-11, Vol.154 (4), p.326-332
Main Authors: Liu, Michelle, Tee, Catherine, Zeng, Fanxing, Sherry, James P., Dixon, Brian, Bols, Niels C., Duncker, Bernard P.
Format: Article
Language:English
Subjects:
Animals
apoptosis
Biomarker
Bleomycin - pharmacology
brain
Brain - metabolism
cell cycle
Cell Cycle Checkpoints - drug effects
Cell Line
Checkpoint
cyclin-dependent kinase
DNA
DNA Damage
DNA repair
Gene Expression
gills
Gills - metabolism
humans
hydroxyurea
Hydroxyurea - pharmacology
mutation
neoplasms
Oncorhynchus mykiss
Oncorhynchus mykiss - metabolism
p53
physiology
polyclonal antibodies
Rainbow Trout
ribonucleotide reductase
tissues
trout
Tumor Suppressor Protein p53 - biosynthesis
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The tumour suppressor protein p53 is a critical component of cell cycle checkpoint responses. It upregulates the expression of cyclin-dependent kinase inhibitors in response to DNA damage and other cellular perturbations, and promotes apoptosis when DNA repair pathways are overwhelmed. Given the high incidence of p53 mutations in human cancers, it has been extensively studied, though only a small fraction of these investigations have been in non-mammalian systems. For the present study, an anti-rainbow trout p53 polyclonal antibody was generated. A variety of rainbow trout (Oncorhynchus mykiss) tissues and cell lines were examined through western blot analysis of cellular protein extracts, which revealed relatively high p53 levels in brain and gills. To evaluate the checkpoint response of rainbow trout p53, RTbrain-W1 and RTgill-W1 cell lines were exposed to varying concentrations of the DNA damaging agent bleomycin and ribonucleotide reductase inhibitor hydroxyurea. In contrast to mammals, these checkpoint-inducing agents provoked no apparent increase in rainbow trout p53 levels. These results infer the presence of alternate DNA damage checkpoint mechanisms in rainbow trout cells.
ISSN:1532-0456
1878-1659
DOI:10.1016/j.cbpc.2011.06.018