Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders

The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from c.c.pro (c.c.pro), Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary record 2011-11, Vol.169 (19), p.495-495
Main Authors: Khan, I., Wieler, L. H., Melzer, F., Gwida, M., Santana, V. L. de. A., de Souza, M. M. A., Saqib, M., Elschner, M. C., Neubauer, H.
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, Bacterial
Burkholderia mallei - immunology
Complement Fixation Tests - standards
Complement Fixation Tests - veterinary
Equidae
Glanders - blood
Glanders - diagnosis
Horse Diseases - blood
Horse Diseases - diagnosis
Horses
Nematodes
Rabbits
Reproducibility of Results
Risk factors
Sensitivity and Specificity
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from c.c.pro (c.c.pro), Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from glanders-endemic and non-endemic areas (200 true-negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and culture-positive (44) horses, donkeys and mules). Immunoblotting (IB) was used as the gold standard test. Highest sensitivity was shown for the CIDC antigen (100 per cent) followed by the c.c.pro antigen (99.39 per cent). However, the USDA antigen showed substantially less (p
ISSN:0042-4900
2042-7670
DOI:10.1136/vr.d5410