Generation of mice with a conditional allele for the p75(NTR) neurotrophin receptor gene

The p75(NTR) neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75(NTR) , many details and aspects remain to be determined. This is in part because the two existing...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2011-11, Vol.49 (11), p.862-869
Main Authors: Bogenmann, Emil, Thomas, Penny S, Li, Qianfeng, Kim, Jieun, Yang, Liang-Tung, Pierchala, Brian, Kaartinen, Vesa
Format: Article
Language:English
Subjects:
Alleles
Animals
Cloning, Molecular
Crosses, Genetic
Embryo, Mammalian - metabolism
Embryo, Mammalian - pathology
Embryonic Development
Exons
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Genotype
Immunohistochemistry
Lower Extremity - physiology
Male
Mice
Mice, Knockout - embryology
Mice, Knockout - genetics
Mice, Knockout - metabolism
Neural Crest - embryology
Neural Crest - metabolism
Neural Crest - pathology
Peripheral Nervous System Diseases - pathology
Receptors, Nerve Growth Factor - genetics
Receptors, Nerve Growth Factor - metabolism
Reflex, Abnormal
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Summary:The p75(NTR) neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75(NTR) , many details and aspects remain to be determined. This is in part because the two existing knockout mouse models (Exons 3 or 4 deleted, respectively), both display features that defy definitive conclusions. Here we describe the generation of mice that carry a conditional p75(NTR) (p75(NTR-FX) ) allele made by flanking Exons 4-6, which encode the transmembrane and all cytoplasmic domains, by loxP sites. To validate this novel conditional allele, both neural crest-specific p75(NTR) /Wnt1-Cre mutants and conventional p75(NTR) null mutants were generated. Both mutants displayed abnormal hind limb reflexes, implying that loss of p75(NTR) in neural crest-derived cells causes a peripheral neuropathy similar to that seen in conventional p75(NTR) mutants. This novel conditional p75(NTR) allele will offer new opportunities to investigate the role of p75(NTR) in specific tissues and cells.
ISSN:1526-968X
DOI:10.1002/dvg.20747