Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine

• Published in: International endodontic journal 2011-12, Vol.44 (12), p.1081-1087
• Main Authors: Han, L., Okiji, T.
• Format: Article
• Language: English
• Subjects: Aluminum Compounds - chemistry; Animals; bioactivity; Biodentine; Calcium; Calcium - analysis; Calcium - pharmacokinetics; Calcium Compounds - chemistry; Calcium influx; Carbon - analysis; Cattle; Crystallization; Data processing; Dental Pulp Cavity - metabolism; Dental Pulp Cavity - ultrastructure; Dentin - metabolism; Dentin - ultrastructure; Dentistry; Deposits; Diffusion; Drug Combinations; Electron probe; Electron Probe Microanalysis; element uptake in dentine; Humidity; Hydrogen-Ion Concentration; Image Processing, Computer-Assisted; Immersion; Incisors; Ionizing radiation; Microscopy, Electron, Scanning; mineral trioxide aggregate; Minerals; Oxides - chemistry; Oxygen - analysis; Phosphorus - analysis; Root Canal Filling Materials - chemistry; Root Canal Preparation - methods; root canals; Roots; Silicates - chemistry; Silicon; Silicon - pharmacokinetics; Spectrometry, X-Ray Emission; Spectroscopy; Time Factors; Ultrastructure; Water - chemistry
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2011.01924.x

Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate–based endodontic materials into root canal dentine. International Endodontic Journal, 44, 1081–1087, 2011. Aim  To compare Biodentine and White ProRoot mineral trioxide aggregate (MTA) with regard to Ca and Si uptake by adjacent root canal dentine in the presence of phosphate‐buffered saline (PBS). Methodology  Root canals of bovine incisor root segments were instrumented, filled with either Biodentine or MTA (n = 20 each) and then immersed in Ca‐and Mg‐free PBS for 1, 7, 30 or 90 days (n = 5 each). Unfilled, unimmersed dentine specimens (n = 5) served as controls. The specimens were sectioned longitudinally, and the ultrastructure of the dentine–material interface and the elemental composition/distribution in the material–adjacent dentine were analysed using a wavelength‐dispersive X‐ray spectroscopy electron probe microanalyser with image observation function. Data were statistically analyzed using one‐way anova and Tukey’s honestly significant difference test or the Mann–Whitney U‐test. Results  Along the material–dentine interface, both materials formed a tag‐like structure that was composed of either Ca‐ and P‐rich crystalline deposits or the material itself. The width of a Ca‐ and Si‐rich layer detected along the dentine layer of the material–dentine interface showed increases over time. The Ca‐ and Si‐rich layer width was significantly larger (P