Initial fibroblast attachment to Erbium:YAG laser-irradiated dentine

• Published in: International endodontic journal 2011-12, Vol.44 (12), p.1134-1144
• Main Authors: Bolortuya, G., Ebihara, A., Ichinose, S., Watanabe, S., Anjo, T., Kokuzawa, C., Saegusa, H., Kawashima, N., Suda, H.
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Animals; Cell Adhesion - physiology; cell behaviour; Cell Count; Cell Culture Techniques; Cell Shape; Chelating Agents - pharmacology; Citric Acid - pharmacology; Dentin - drug effects; Dentin - radiation effects; Dentin - ultrastructure; Dentistry; Doxycycline - pharmacology; Edetic Acid - pharmacology; endodontic treatment; Er:YAG laser; Fibroblasts - physiology; Humans; Lasers, Solid-State - therapeutic use; Low-Level Light Therapy - methods; Mice; Microscopy, Electron, Scanning; Peroxides - pharmacology; Polysorbates - pharmacology; Radiation Dosage; resected root surface; Root Canal Irrigants - pharmacology; root-end resection; Time Factors; Urea - pharmacology; Waxes - pharmacology
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2011.01934.x

Bolortuya G, Ebihara A, Ichinose S, Watanabe S, Anjo T, Kokuzawa C, Saegusa H, Kawashima N, Suda H. Initial fibroblast attachment to Erbium:YAG laser‐irradiated dentine. International Endodontic Journal, 44, 1134–1144, 2011. Aims  To evaluate the effects of Erbium (Er):YAG laser irradiation on the morphology of resected dentine surfaces, and to investigate fibroblast attachment to laser‐irradiated dentine surfaces. Methodology  Dentine blocks obtained from single‐rooted human teeth were divided into the following groups after sterilization in an autoclave: (i) Laser group treated with Er:YAG laser irradiation (30 mJ per pulse, 10 pps, 60 s); (ii) L‐MTAD group treated with laser irradiation as in (i) plus a mixture of doxycycline, tetracycline isomer and citric acid; (iii) RC‐Prep group treated with EDTA gel or cream (RC‐Prep) and (iv) Control group left untreated. After each treatment, the dentine blocks were incubated with NIH/3T3 fibroblasts cultured to subconfluency in Dulbecco’s modified Eagle’s medium supplemented with 10% foetal bovine serum and antibiotics. The number of attached cells amongst the groups was analysed statistically at the 5% significance level. The dentine surface morphologies and cell attachments were evaluated by counting assays, histological observations and scanning electron microscopy (SEM). Results  The number of attached cells was significantly higher (P