Bacterial diversity of periodontal and implant-related sites detected by the DNA Checkerboard method

The aim of this study was to compare the microbial composition of the subgingival biofilm from teeth and implant sulci in relation to contents originating from internal parts of the implant, abutment and implant prosthesis. Twenty subgingival biofilm samples from the mesial and distal aspects of eac...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2011-12, Vol.30 (12), p.1607-1613
Main Authors: do Nascimento, C., Monesi, N., Ito, I. Y., Issa, J. P. M., de Albuquerque Junior, R. F.
Format: Article
Language:English
Subjects:
Adult
Aged
Bacteria
Bacteria - classification
Bacteria - genetics
Bacterial Load
Biodiversity
Biofilms
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Capnocytophaga gingivalis
Correlation analysis
Dental implants
Dentistry
Deoxyribonucleic acid
DNA
Edentulous
Female
Humans
Infectious diseases
Internal Medicine
Male
Medical dressings
Medical Microbiology
Medical sciences
Microbiota
Middle Aged
Periodontal Pocket - microbiology
Periodontal Prosthesis - microbiology
Prevotella intermedia
Prostheses
Teeth
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Summary:The aim of this study was to compare the microbial composition of the subgingival biofilm from teeth and implant sulci in relation to contents originating from internal parts of the implant, abutment and implant prosthesis. Twenty subgingival biofilm samples from the mesial and distal aspects of each tooth/implant and 29 samples from the internal parts of titanium implants, abutments and implant prostheses were evaluated for the presence of 18 bacterial species using DNA Checkerboard and the differences between samples from teeth and implants were assessed with Pearson’s correlation analysis. The periodontal and peri-implantar sulci presented significantly higher bacterial counts than the implant-related sites ( p  
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-011-1267-1