Electrophoretic Time-of-Flight Measurements of Single DNA Molecules with Two Stacked Nanopores

Electrophoretic transport through a solid-state nanodevice comprised of two stacked nanopore sensors is used to determine the free-solution mobility of DNA molecules based on their “time-of-flight” between the two pores. Mobility measurements are possible at very low (100 pM) DNA concentration and f...

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Bibliographic Details
Published in:Nano letters 2011-11, Vol.11 (11), p.5002-5007
Main Authors: Langecker, Martin, Pedone, Daniel, Simmel, Friedrich C, Rant, Ulrich
Format: Article
Language:English
Subjects:
Applied sciences
Biosensing Techniques - instrumentation
Condensed matter: structure, mechanical and thermal properties
Deoxyribonucleic acid
Devices
DNA - analysis
DNA - chemistry
Dynamic tests
Electronics
Electrophoresis - instrumentation
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
Gain
General equipment and techniques
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Molecular electronics, nanoelectronics
Nanostructure
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - instrumentation
Physics
Polymers
Porosity
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Sensors (chemical, optical, electrical, movement, gas, etc.)
remote sensing
Statistical analysis
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Transport
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Description
Summary:Electrophoretic transport through a solid-state nanodevice comprised of two stacked nanopore sensors is used to determine the free-solution mobility of DNA molecules based on their “time-of-flight” between the two pores. Mobility measurements are possible at very low (100 pM) DNA concentration and for low as well as high salt concentrations (here 30 mM and 1 M KCl). The mechanism of DNA transport through the device is elucidated by statistical analysis, showing the free-draining nature of the translocating DNA polymers and a barrier-dominated escape through the second pore. Furthermore, consecutive threading of single molecules through the two pores can be used to gain more detailed information on the dynamics of the molecules by correlation analysis, which also provides a direct electrical proof for translocation.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl2030079