Highly efficient peptide separations in proteomics Part 2: Bi-and multidimensional liquid-based separation techniques

Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-04, Vol.877 (11-12), p.1019-1039
Main Authors: SANDRA, Koen, MOSHIR, Mahan, D'HONDT, Filip, TUYTTEN, Robin, VERLEYSEN, Katleen, KAS, Koen, FRANCOIS, Isabelle, SANDRA, Pat
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Electrophoresis, Capillary
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Isoelectric Focusing
Medical sciences
Peptides - isolation & purification
Pharmacology. Drug treatments
Proteomics - methods
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2009.02.050