Doubled haploid production in Flax ( Linum usitatissimum L.)

There is a requirement of haploid and double haploid material and homozygous lines for cell culture studies and breeding in flax. Anther culture is currently the most successful method producing doubled haploid lines in flax. Recently, ovary culture was also described as a good source of doubled hap...

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Bibliographic Details
Published in:Biotechnology advances 2009-07, Vol.27 (4), p.371-375
Main Authors: Obert, Bohuš, Žáčková, Zuzana, Šamaj, Jozef, Preťová, Anna
Format: Article
Language:English
Subjects:
Anther culture
Biological and medical sciences
Biomarkers - analysis
Biotechnology
Biotechnology - methods
Cell Culture Techniques
Doubled haploid
Flax - genetics
Flax - growth & development
Flowers - genetics
Flowers - growth & development
Flowers - physiology
Fundamental and applied biological sciences. Psychology
Haploid
Haploidy
Linum usitatissimum
Microspore culture
Ovule culture
Ploidy level
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Summary:There is a requirement of haploid and double haploid material and homozygous lines for cell culture studies and breeding in flax. Anther culture is currently the most successful method producing doubled haploid lines in flax. Recently, ovary culture was also described as a good source of doubled haploids. In this review we focus on tissue and plants regeneration using anther culture, and cultivation of ovaries containing unfertilized ovules. The effect of genotype, physiological status of donor plants, donor material pre-treatment and cultivation conditions for flax anthers and ovaries is discussed here. The process of plant regeneration from anther and ovary derived calli is also in the focus of this review. Attention is paid to the ploidy level of regenerated tissue and to the use of molecular markers for determining of gametic origin of flax plants derived from anther and ovary cultures. Finally, some future prospects on the use of doubled haploids in flax biotechnology are outlined here.
ISSN:0734-9750
1873-1899
DOI:10.1016/j.biotechadv.2009.02.004