Antimicrobial effects of an NO-releasing poly(ethylene vinylacetate) coating on soft-tissue implants in vitro and in a murine model

• Published in: Acta biomaterialia 2009-07, Vol.5 (6), p.1905-1910
• Main Authors: Engelsman, Anton F., Krom, Bastiaan P., Busscher, Henk J., van Dam, Gooitzen M., Ploeg, Rutger J., van der Mei, Henny C.
• Format: Article
• Language: English
• Subjects: Abdominal wall reconstruction; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemistry; Biofilm; Connective Tissue - surgery; Diffusion; Drug Implants - administration & dosage; Drug Implants - chemistry; Escherichia coli; Female; In vivo imaging; Mesh infection; Mice; Mice, Inbred BALB C; Monofilament polypropylene mesh; Nitric Oxide - administration & dosage; Nitric Oxide - chemistry; Polyvinyls - chemistry; Prostheses and Implants; Pseudomonas aeruginosa; Staphylococcal Infections - drug therapy; Staphylococcus aureus
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2009.01.041

Infection of surgical meshes used in abdominal wall reconstructions often leads to removal of the implant and increases patient morbidity due to repetitive operations and hospital administrations. Treatment with antibiotics is ineffective due to the biofilm mode of growth of the infecting bacteria and bears the risk of inducing antibiotic resistance. Hence there is a need for alternative methods to prevent and treat mesh infection. Nitric oxide (NO)-releasing coatings have been demonstrated to possess bactericidal properties in vitro. It is the aim of this study to assess possible benefits of a low concentration NO-releasing carbon-based coating on monofilament polypropylene meshes with respect to infection control in vitro and in vivo. When applied on surgical meshes, NO-releasing coatings showed significant bactericidal effect on in vitro biofilms of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and CNS. However, using bioluminescent in vivo imaging, no beneficial effects of this NO-releasing coating on subcutaneously implanted surgical meshes in mice could be observed.