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The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs ( Potamonautes warreni)
Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab, Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02 μmol g −1 wet mass±0.02), Cu (0.326 μmol g −1 wet mass±0.15), and Zn (0.534 μmol g −1 wet mass±0.20). The native prote...
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| Published in: | Ecotoxicology and environmental safety 2009-05, Vol.72 (4), p.1220-1227 |
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| Main Authors: | , , |
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| cites | cdi_FETCH-LOGICAL-c454t-50fe633c732058cf2868ae06aabcebb3b4347d40343c3df095f1172cca4e040c3 |
| container_end_page | 1227 |
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| container_title | Ecotoxicology and environmental safety |
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| creator | Schuwerack, P.-M.M. Lewis, John W. Jones, Peter |
| description | Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab,
Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02
μmol
g
−1 wet mass±0.02), Cu (0.326
μmol
g
−1 wet mass±0.15), and Zn (0.534
μmol
g
−1 wet mass±0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (
M) of 9.10
kDa±1.74, 8.95
kDa±1.66, and 9.32
kDa±0.93. With exposure to 0.2
mg
Cd
2+
l
−1 for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54
kDa±1.64), coinciding with a Zn-bound MT-like component (8.2
kDa±1.54). Less than 10% were bound in the high
M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8
kDa) with a predominant Cu-binding component. |
| doi_str_mv | 10.1016/j.ecoenv.2008.07.004 |
| format | article |
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Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02
μmol
g
−1 wet mass±0.02), Cu (0.326
μmol
g
−1 wet mass±0.15), and Zn (0.534
μmol
g
−1 wet mass±0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (
M) of 9.10
kDa±1.74, 8.95
kDa±1.66, and 9.32
kDa±0.93. With exposure to 0.2
mg
Cd
2+
l
−1 for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54
kDa±1.64), coinciding with a Zn-bound MT-like component (8.2
kDa±1.54). Less than 10% were bound in the high
M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8
kDa) with a predominant Cu-binding component.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2008.07.004</identifier><identifier>PMID: 18992941</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Biomarkers ; Brachyura - metabolism ; Cadmium - metabolism ; Cadmium - toxicity ; Copper - metabolism ; Cytosol - metabolism ; Decapoda ; Digestive gland ; Digestive System - metabolism ; Dynamics metal and protein metabolism ; Female ; Fresh Water ; Freshwater ; Freshwater crab Potamonautes warreni ; Integrated molecular responses to Cd ; Liver - metabolism ; Male ; Metalloproteins - chemistry ; Metalloproteins - metabolism ; Metals - metabolism ; Potamonautes warreni ; Protein Binding ; Water Pollutants, Chemical - toxicity ; Zinc - metabolism</subject><ispartof>Ecotoxicology and environmental safety, 2009-05, Vol.72 (4), p.1220-1227</ispartof><rights>2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-50fe633c732058cf2868ae06aabcebb3b4347d40343c3df095f1172cca4e040c3</citedby><cites>FETCH-LOGICAL-c454t-50fe633c732058cf2868ae06aabcebb3b4347d40343c3df095f1172cca4e040c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147651308001863$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3535,27903,27904,45759</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18992941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schuwerack, P.-M.M.</creatorcontrib><creatorcontrib>Lewis, John W.</creatorcontrib><creatorcontrib>Jones, Peter</creatorcontrib><title>The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs ( Potamonautes warreni)</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab,
Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02
μmol
g
−1 wet mass±0.02), Cu (0.326
μmol
g
−1 wet mass±0.15), and Zn (0.534
μmol
g
−1 wet mass±0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (
M) of 9.10
kDa±1.74, 8.95
kDa±1.66, and 9.32
kDa±0.93. With exposure to 0.2
mg
Cd
2+
l
−1 for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54
kDa±1.64), coinciding with a Zn-bound MT-like component (8.2
kDa±1.54). Less than 10% were bound in the high
M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8
kDa) with a predominant Cu-binding component.</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Brachyura - metabolism</subject><subject>Cadmium - metabolism</subject><subject>Cadmium - toxicity</subject><subject>Copper - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Decapoda</subject><subject>Digestive gland</subject><subject>Digestive System - metabolism</subject><subject>Dynamics metal and protein metabolism</subject><subject>Female</subject><subject>Fresh Water</subject><subject>Freshwater</subject><subject>Freshwater crab Potamonautes warreni</subject><subject>Integrated molecular responses to Cd</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Metalloproteins - chemistry</subject><subject>Metalloproteins - metabolism</subject><subject>Metals - metabolism</subject><subject>Potamonautes warreni</subject><subject>Protein Binding</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Zinc - metabolism</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi1ERbeFN0DIJyiHhHHsOMmlEloVqFQJDuVsOeOJ6lUSL3a2pW9fb3clbuViy_Y3_8jzMfZeQClA6C-bkjDQfF9WAG0JTQmgXrGVgA6KSgn1mq1AqKbQtZCn7CylDQBIqOs37FS0XVd1SqxYvL0j7h5nO3lMPAx8G8NCfuZ2dnyixY7Pax9Gnya-v0cc_WQXcs_I2hX0dxtSPg6R0t1Dfokco-0Tv-C_wmKnMNvdQok_2Bhp9p_fspPBjoneHfdz9vvb1e36R3Hz8_v1-utNgapWS1HDQFpKbGQFdYtD1erWEmhre6S-l72SqnEKpJIo3QBdPQjRVIhWEShAec4-HXLzl_7sKC1m8glpHO1MYZdMB1Ir0egukx9fJKVSugNd_ReshBBtBftEdQAxhpQiDWYb89TioxFg9vrMxhz0mb0-A43J-nLZh2P-rp_I_Ss6-srA5QGgPLh7T9Ek9DQjOR8JF-OCf7nDE8Qkrek</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Schuwerack, P.-M.M.</creator><creator>Lewis, John W.</creator><creator>Jones, Peter</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20090501</creationdate><title>The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs ( Potamonautes warreni)</title><author>Schuwerack, P.-M.M. ; Lewis, John W. ; Jones, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-50fe633c732058cf2868ae06aabcebb3b4347d40343c3df095f1172cca4e040c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biomarkers</topic><topic>Brachyura - metabolism</topic><topic>Cadmium - metabolism</topic><topic>Cadmium - toxicity</topic><topic>Copper - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Decapoda</topic><topic>Digestive gland</topic><topic>Digestive System - metabolism</topic><topic>Dynamics metal and protein metabolism</topic><topic>Female</topic><topic>Fresh Water</topic><topic>Freshwater</topic><topic>Freshwater crab Potamonautes warreni</topic><topic>Integrated molecular responses to Cd</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Metalloproteins - chemistry</topic><topic>Metalloproteins - metabolism</topic><topic>Metals - metabolism</topic><topic>Potamonautes warreni</topic><topic>Protein Binding</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schuwerack, P.-M.M.</creatorcontrib><creatorcontrib>Lewis, John W.</creatorcontrib><creatorcontrib>Jones, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schuwerack, P.-M.M.</au><au>Lewis, John W.</au><au>Jones, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs ( Potamonautes warreni)</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>72</volume><issue>4</issue><spage>1220</spage><epage>1227</epage><pages>1220-1227</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><abstract>Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab,
Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02
μmol
g
−1 wet mass±0.02), Cu (0.326
μmol
g
−1 wet mass±0.15), and Zn (0.534
μmol
g
−1 wet mass±0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (
M) of 9.10
kDa±1.74, 8.95
kDa±1.66, and 9.32
kDa±0.93. With exposure to 0.2
mg
Cd
2+
l
−1 for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54
kDa±1.64), coinciding with a Zn-bound MT-like component (8.2
kDa±1.54). Less than 10% were bound in the high
M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8
kDa) with a predominant Cu-binding component.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>18992941</pmid><doi>10.1016/j.ecoenv.2008.07.004</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0147-6513 |
| ispartof | Ecotoxicology and environmental safety, 2009-05, Vol.72 (4), p.1220-1227 |
| issn | 0147-6513 1090-2414 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_903641769 |
| source | ScienceDirect Freedom Collection; ScienceDirect - Connect here FIRST to enable access |
| subjects | Animals Biomarkers Brachyura - metabolism Cadmium - metabolism Cadmium - toxicity Copper - metabolism Cytosol - metabolism Decapoda Digestive gland Digestive System - metabolism Dynamics metal and protein metabolism Female Fresh Water Freshwater Freshwater crab Potamonautes warreni Integrated molecular responses to Cd Liver - metabolism Male Metalloproteins - chemistry Metalloproteins - metabolism Metals - metabolism Potamonautes warreni Protein Binding Water Pollutants, Chemical - toxicity Zinc - metabolism |
| title | The dynamics of protein and metal metabolism in acclimated and Cd-exposed freshwater crabs ( Potamonautes warreni) |
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