Simplified procedures for the isolation of HF3, bothropasin, disintegrin-like/cysteine-rich protein and a novel P-I metalloproteinase from Bothrops jararaca venom

HF3 and bothropasin are P-III hemorrhagic snake venom metalloproteinases (SVMPs) of Bothrops jararaca. The DC protein is composed of the disintegrin-like/cysteine-rich domains derived from the autolysis of P-III SVMPs. Here we describe simplified procedures for the isolation of HF3, bothropasin, the...

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Bibliographic Details
Published in:Toxicon (Oxford) 2009-06, Vol.53 (7), p.797-801
Main Authors: Oliveira, Ana K., Paes Leme, Adriana F., Assakura, Marina T., Menezes, Milene C., Zelanis, André, Tashima, Alexandre K., Lopes-Ferreira, Mônica, Lima, Carla, Camargo, Antonio C.M., Fox, Jay W., Serrano, Solange M.T.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Bothrops - physiology
Bothrops jararaca
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Crotalid Venoms - isolation & purification
Crotalid Venoms - toxicity
Electrophoresis, Polyacrylamide Gel
Fibrinogenolysis
Hemorrhage
Hemorrhage - chemically induced
Hemorrhage - pathology
Medical sciences
Metalloendopeptidases - isolation & purification
Metalloendopeptidases - toxicity
Metalloproteases - isolation & purification
Metalloproteases - toxicity
Mice
Molecular Sequence Data
Proteolysis
Snake venom metalloproteinase
Toxicology
Trypsin - chemistry
Viper Venoms - enzymology
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Summary:HF3 and bothropasin are P-III hemorrhagic snake venom metalloproteinases (SVMPs) of Bothrops jararaca. The DC protein is composed of the disintegrin-like/cysteine-rich domains derived from the autolysis of P-III SVMPs. Here we describe simplified procedures for the isolation of HF3, bothropasin, the DC protein, and BJ-PI, a novel P-I SVMP. The isolated proteins were identified by mass spectrometry. BJ-PI is a potent caseinolytic enzyme devoid of hemorrhagic activity. HF3, bothropasin and BJ-PI show distinct fibrinogenolytic activities.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2009.02.019