The defined presentation of nanoparticles to cells and their surface controlled uptake

Abstract New approaches and standardized test procedures to study the impact of nanoparticles (NPs) on living cells are urgently needed for the evaluation of potential hazards relating human exposure to NPs. Here we study semiconductor quantum dots (QDs) preparation on solid surfaces and the subsequ...

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Bibliographic Details
Published in:Biomaterials 2009-08, Vol.30 (22), p.3766-3770
Main Authors: Alberola, Alicia Piera, Rädler, Joachim O
Format: Article
Language:English
Subjects:
Advanced Basic Science
Biocompatible Materials - metabolism
Cell Nucleus - metabolism
Cells - cytology
Cells - metabolism
Dentistry
ECM
Endocytosis - physiology
Epithelial cell
Humans
Image analysis
Materials Testing
Nanoparticle
Nanoparticles
Nanotoxicity
Particle Size
Quantum Dots
Semiconductors
Surface Properties
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Summary:Abstract New approaches and standardized test procedures to study the impact of nanoparticles (NPs) on living cells are urgently needed for the evaluation of potential hazards relating human exposure to NPs. Here we study semiconductor quantum dots (QDs) preparation on solid surfaces and the subsequent internalization by cells seeded on top of the NP layer. Controlled densities of NPs are adsorbed to solid surfaces coated with extra-cellular macromolecules. The fraction of QDs taken up by the cells is assessed by automated fluorescence microscopy z -scans. We find that NPs aggregate during the uptake process, forming clusters inside cells that are able to enter the cell nucleus. NP uptake is dependent on surface functionalization and can be hindered by increasing the strength of the adhesion force between NPs and the surface. Studying time dependent uptake, we show that particles are able to exit cells.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2009.03.031