Sol―gel derived nano-structured zinc oxide film for sexually transmitted disease sensor

A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disea...

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Bibliographic Details
Published in:Analyst (London) 2009-05, Vol.134 (5), p.997-1002
Main Authors: ANSARI, Anees A, SINGH, Renu, SUMANA, G, MALHOTRA, B. D
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Electrochemical methods
Electrochemical Techniques - instrumentation
Electrochemical Techniques - methods
Electrodes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Gonorrhea - diagnosis
Humans
Methods. Procedures. Technologies
Nanostructures - chemistry
Neisseria gonorrhoeae
Neisseria gonorrhoeae - isolation & purification
Phase Transition
Spectrometric and optical methods
Various methods and equipments
Zinc Oxide - chemistry
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Summary:A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA-ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol-0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.
ISSN:0003-2654
1364-5528
DOI:10.1039/b817562d