Polymorphisms in the P450 c17 (17-Hydroxylase/17, 20-Lyase) Gene: Association With Estradiol and Testosterone Concentration in Hypospadias

• Published in: Urology (Ridgewood, N.J.) N.J.), 2011-10, Vol.78 (4), p.902-907
• Main Authors: Yadav, Chandra Shekhar, Bajpai, Minu, Kumar, Vivek, Datta, Sudip Kumar, Gupta, Piyush, Ahmed, Rafat Sultana, Banerjee, Basu Dev
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Case-Control Studies; Child; Child, Preschool; Estradiol - blood; Genotype; Gynecology. Andrology. Obstetrics; Homozygote; Humans; Hypospadias - blood; Hypospadias - genetics; Infant; Male; Male genital diseases; Malformations of the urinary system; Medical sciences; Nephrology. Urinary tract diseases; Non tumoral diseases; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Steroid 17-alpha-Hydroxylase - blood; Steroid 17-alpha-Hydroxylase - genetics; Testosterone - blood; Urinary tract. Prostate gland; Urology
• ISSN: 0090-4295; 1527-9995
• DOI: 10.1016/j.urology.2011.04.021

Objective To investigate the association of CYP17 polymorphism with 17β-estradiol (E2) and testosterone (T) concentration in hypospadias. Methods Two-hundred twenty-three boys (91 with hypospadias and 132 age-matched controls) were included in the study. CYP17 polymorphism was evaluated using the polymerase chain reaction-restriction fragment length polymorphism method, whereas T and E2 levels were estimated in serum by enzyme-linked immunosorbent assay. Association between CYP17 genotypes and 17β-E2, T, and their ratio (E2/T) was analyzed by analysis of variance followed by Tukey's test. 17β-E2, T, and E2/T ratio was also compared among the different degrees of hypospadias, as well as in controls, by unpaired Student's t -test. Results Significantly low levels of T were observed in severe-degree hypospadias (n = 14; mean ± SD = 1.01 ± 0.57) compared with mild cases (n = 77; mean ± SD = 1.93 ± 1.40) and controls (mean ± SD = 3.32 ± 2.06) ( P