CTGF/CCN2 has a chemoattractive function but a weak adhesive property to embryonic carcinoma cells

► We studied the chemotactic and adhesive roles of CCN2 to embryonic carcinoma cells. ► CCN2 showed high chemotactic effects for P19 cells. ► CCN2 is a weak adhesive molecule for P19 cells. ► We created a method to measure molecular adhesive properties under different cellular conditions. Connective...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2011-10, Vol.413 (4), p.582-587
Main Authors: Aguiar, Diego P., Pontes, Bruno, Mendes, Fabio A., Andrade, Leonardo R., Viana, Nathan B., Abreu, José G.
Format: Article
Language:English
Subjects:
Adhesion
Animals
Bovine serum albumin
Boyden chamber
CCN2
Cell Adhesion
Cell Line, Tumor
Cell migration
Cell Proliferation
Chemoattraction
Chemotaxis
Connective tissue growth factor
Connective Tissue Growth Factor - pharmacology
Connective Tissue Growth Factor - physiology
Embryonal Carcinoma Stem Cells - metabolism
Embryonal Carcinoma Stem Cells - pathology
Embryos
Fibronectin
Laminin
Mice
Migration
Optical tweezers
P19 cells
polystyrene
Sepharose - chemistry
Teratocarcinoma
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Summary:► We studied the chemotactic and adhesive roles of CCN2 to embryonic carcinoma cells. ► CCN2 showed high chemotactic effects for P19 cells. ► CCN2 is a weak adhesive molecule for P19 cells. ► We created a method to measure molecular adhesive properties under different cellular conditions. Connective tissue growth factor (CTGF/CCN2) is a protein of the CCN family that modulates cell–ECM interactions in a variety of cell types. In this study, we investigated the chemotactic and adhesive properties of CCN2 protein in embryonic teratocarcinoma P19 cells. Initially, P19 cells were attracted to CCN2-coated agarose beads. In Boyden chamber experiments, CCN2-containing medium induced a threefold greater migration of P19 cells. CCN2 adhesion properties were studied by using optical tweezers. The specific adhesion times of P19 cells to polystyrene beads coated with laminin, fibronectin, CCN2 and bovine serum albumin were 1.8±0.5s, 2.7±0.4s, 10±2s and 13±2s, respectively, revealing an unexpectedly low adhesive capacity of CCN2 protein for P19 cells. In conclusion, our findings support the chemoattractive role of CCN2 for P19 cells, but not its adhesive role when compared to laminin or fibronectin.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2011.09.005