Rapid species specific identification and subtyping of Yersinia enterocolitica by MALDI-TOF Mass spectrometry

• Published in: Journal of microbiological methods 2011-11, Vol.87 (2), p.150-153
• Main Authors: Stephan, Roger, Cernela, Nicole, Ziegler, Dominik, Pflüger, Valentin, Tonolla, Mauro, Ravasi, Damiana, Fredriksson-Ahomaa, Maria, Hächler, Herbert
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - chemistry; Bacterial Typing Techniques - methods; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; biomarkers; biotypes; foodborne illness; Fundamental and applied biological sciences. Psychology; Identification; MALDI-TOF-MS; matrix-assisted laser desorption-ionization mass spectrometry; Microbiology; molecular weight; pathogens; Sensitivity and Specificity; species identification; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; subtyping; Tandem Mass Spectrometry - methods; Yersinia enterocolitica; Yersinia enterocolitica - chemistry; Yersinia enterocolitica - classification; Yersinia enterocolitica - isolation & purification; Yersinia Infections - diagnosis; Yersinia Infections - microbiology
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2011.08.016

Yersinia enterocolitica are Gram-negative pathogens and known as important causes of foodborne infections. Rapid and reliable identification of strains of the species Y. enterocolitica within the genus Yersinia and the differentiation of the pathogenic from the non-pathogenic biotypes has become increasingly important. We evaluated here the application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid species identification and subtyping of Y. enterocolitica. To this end, we developed a reference MS database library including 19 Y. enterocolitica (non-pathogenic biotype 1A and pathogenic biotypes 2 and 4) as well as 24 non-Y. enterocolitica strains, belonging to eleven different other Yersinia spp. The strains provided reproducible and unique mass spectra profiles covering a wide molecular mass range (2000 to 30,000Da). Species-specific and biotype-specific biomarker protein mass patterns were determined for Y. enterocolitica. The defined biomarker mass patterns (SARAMIS SuperSpectrum™) were validated using 117 strains from various Y. enterocolitica bioserotypes in a blind-test. All strains were correctly identified and for all strains the mass spectrometry-based identification scheme yielded identical results compared to a characterization by a combination of biotyping and serotyping. Our study demonstrates that MALDI-TOF-MS is a reliable and powerful tool for the rapid identification of Y. enterocolitica strains to the species level and allows subtyping of strains to the biotype level. ► Yersinia enterocolitica are known as important causes of foodborne infections. ► MALDI-TOF-MS proofed to be a reliable and powerful identification tool. ► MALDI-TOF-MS allows subtyping of strains to the biotype level.