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Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis

ABSTRACT Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reacti...

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Published in:Plant, cell and environment cell and environment, 2011-11, Vol.34 (11), p.1900-1906
Main Authors: KHOKON, MD. ATIQUR RAHMAN, JAHAN, MD. SARWAR, RAHMAN, TANIYA, HOSSAIN, MOHAMMAD ANOWAR, MUROYAMA, DAICHI, MINAMI, IKUKO, MUNEMASA, SHINTARO, MORI, IZUMI C., NAKAMURA, YOSHIMASA, MURATA, YOSHIYUKI
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cited_by cdi_FETCH-LOGICAL-c5475-c5ad5106e416254ab789aa900d19c6c473c346f2a0030801ef62d0c44cd0c44c3
cites cdi_FETCH-LOGICAL-c5475-c5ad5106e416254ab789aa900d19c6c473c346f2a0030801ef62d0c44cd0c44c3
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container_issue 11
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container_title Plant, cell and environment
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creator KHOKON, MD. ATIQUR RAHMAN
JAHAN, MD. SARWAR
RAHMAN, TANIYA
HOSSAIN, MOHAMMAD ANOWAR
MUROYAMA, DAICHI
MINAMI, IKUKO
MUNEMASA, SHINTARO
MORI, IZUMI C.
NAKAMURA, YOSHIMASA
MURATA, YOSHIYUKI
description ABSTRACT Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca2+. AITC‐induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC‐induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2‐2 and methyl jasmonate (MeJA)‐deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC‐induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata. Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects and herbivores and ITCs are cytotoxic to both animals and plants at the higher dosage. However, physiological significance of ITCs in plants remains unknown. This study demonstrates that allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis and that AITC signaling shares some signal components with ABA signaling and MeJA signaling, production of reactive oxygen species (ROS) and nitric oxide (NO) and elevation of cytosolic Ca2+. Moreover, AITC‐induced stomatal closure requires MeJA priming but not ABA priming.
doi_str_mv 10.1111/j.1365-3040.2011.02385.x
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ATIQUR RAHMAN ; JAHAN, MD. SARWAR ; RAHMAN, TANIYA ; HOSSAIN, MOHAMMAD ANOWAR ; MUROYAMA, DAICHI ; MINAMI, IKUKO ; MUNEMASA, SHINTARO ; MORI, IZUMI C. ; NAKAMURA, YOSHIMASA ; MURATA, YOSHIYUKI</creator><creatorcontrib>KHOKON, MD. ATIQUR RAHMAN ; JAHAN, MD. SARWAR ; RAHMAN, TANIYA ; HOSSAIN, MOHAMMAD ANOWAR ; MUROYAMA, DAICHI ; MINAMI, IKUKO ; MUNEMASA, SHINTARO ; MORI, IZUMI C. ; NAKAMURA, YOSHIMASA ; MURATA, YOSHIYUKI</creatorcontrib><description>ABSTRACT Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca2+. AITC‐induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC‐induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2‐2 and methyl jasmonate (MeJA)‐deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC‐induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata. Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects and herbivores and ITCs are cytotoxic to both animals and plants at the higher dosage. However, physiological significance of ITCs in plants remains unknown. 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ATIQUR RAHMAN</creatorcontrib><creatorcontrib>JAHAN, MD. SARWAR</creatorcontrib><creatorcontrib>RAHMAN, TANIYA</creatorcontrib><creatorcontrib>HOSSAIN, MOHAMMAD ANOWAR</creatorcontrib><creatorcontrib>MUROYAMA, DAICHI</creatorcontrib><creatorcontrib>MINAMI, IKUKO</creatorcontrib><creatorcontrib>MUNEMASA, SHINTARO</creatorcontrib><creatorcontrib>MORI, IZUMI C.</creatorcontrib><creatorcontrib>NAKAMURA, YOSHIMASA</creatorcontrib><creatorcontrib>MURATA, YOSHIYUKI</creatorcontrib><title>Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>ABSTRACT Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. 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ATIQUR RAHMAN</au><au>JAHAN, MD. SARWAR</au><au>RAHMAN, TANIYA</au><au>HOSSAIN, MOHAMMAD ANOWAR</au><au>MUROYAMA, DAICHI</au><au>MINAMI, IKUKO</au><au>MUNEMASA, SHINTARO</au><au>MORI, IZUMI C.</au><au>NAKAMURA, YOSHIMASA</au><au>MURATA, YOSHIYUKI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2011-11</date><risdate>2011</risdate><volume>34</volume><issue>11</issue><spage>1900</spage><epage>1906</epage><pages>1900-1906</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>ABSTRACT Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca2+. AITC‐induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC‐induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2‐2 and methyl jasmonate (MeJA)‐deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC‐induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata. Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects and herbivores and ITCs are cytotoxic to both animals and plants at the higher dosage. However, physiological significance of ITCs in plants remains unknown. This study demonstrates that allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis and that AITC signaling shares some signal components with ABA signaling and MeJA signaling, production of reactive oxygen species (ROS) and nitric oxide (NO) and elevation of cytosolic Ca2+. Moreover, AITC‐induced stomatal closure requires MeJA priming but not ABA priming.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21711355</pmid><doi>10.1111/j.1365-3040.2011.02385.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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ispartof Plant, cell and environment, 2011-11, Vol.34 (11), p.1900-1906
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1365-3040
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source Wiley-Blackwell Read & Publish Collection
subjects [Ca2+]cyt oscillation
Abscisic acid
Acetates - pharmacology
Arabidopsis
Arabidopsis - drug effects
Arabidopsis - physiology
Biological and medical sciences
Calcium - metabolism
Cyclopentanes - pharmacology
Cytoplasm - drug effects
Cytoplasm - metabolism
Fundamental and applied biological sciences. Psychology
glutathione
Glutathione - pharmacology
Isothiocyanates - pharmacology
Movement - drug effects
Mutation - genetics
myrosinases
Nitric Oxide - metabolism
Oxylipins - pharmacology
Plant Stomata - cytology
Plant Stomata - drug effects
Plant Stomata - physiology
Reactive Oxygen Species - metabolism
ROS
title Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis
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