Regeneration of Tetrabutylammonium Ion-Pairing Reagent Distribution in a Gradient Elution of Reversed Phase Ion-Pair Chromatography

The regeneration of ion-pairing reagent distribution on liquid chromatography columns after gradient elution has been well recognized as the cause for long column equilibration time, a major drawback associated with gradient elution reverse phase ion-pair chromatography. To date, the majority of stu...

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Bibliographic Details
Published in:Journal of chromatographic science 2011-11, Vol.49 (10), p.825-831
Main Authors: Zhang, Jin, Raglione, Thomas, Wang, Qinggang, Kleintop, Brent, Tomasella, Frank, Liang, Xiaozhong
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange - instrumentation
Chromatography, Ion Exchange - methods
Chromatography, Reverse-Phase
Exact sciences and technology
Indicators and Reagents - chemistry
Linear Models
Other chromatographic methods
Quaternary Ammonium Compounds - chemistry
Time Factors
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Summary:The regeneration of ion-pairing reagent distribution on liquid chromatography columns after gradient elution has been well recognized as the cause for long column equilibration time, a major drawback associated with gradient elution reverse phase ion-pair chromatography. To date, the majority of studies have focused on optimizing the separation conditions to shorten the equilibration time. There is limited understanding of the ion-pairing reagent distribution process between the mobile phase and stationary phase in the course of gradient elution, and subsequent column re-equilibration. The focus of this work is to gain a better understanding of this process. An ion-pair Chromatographic system, equipped with a YMC ODS C18 column and a mobile phase containing tetrabutylammonium (TBA) hydroxide as the ion-pairing reagent, was used in the study. The TBA distribution profile was established by measuring its concentration in the eluent fractions collected during the gradient cycle using different column equilibration times with an ion Chromatographic method. Furthermore, the analyte retention time was evaluated as the function of the column equilibration time and TBA concentration in the mobile phase. The column equilibration and its impact on the method robustness will also be discussed.
ISSN:0021-9665
1945-239X
DOI:10.1093/chrsci/49.10.825