Sequential Injection Immunoassay for Environmental Measurements

Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because...

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Bibliographic Details
Published in:Analytical Sciences 2011/11/10, Vol.27(11), pp.1069-1069
Main Authors: SOH, Nobuaki, TANAKA, Mayumi, HIRAKAWA, Koji, ZHANG, RuiQi, NAKAJIMA, Hizuru, NAKANO, Koji, IMATO, Toshihiko
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Biomarkers - analysis
Biomarkers - metabolism
Chemistry
Environmental Monitoring - instrumentation
Environmental Monitoring - methods
Enzyme-Linked Immunosorbent Assay - instrumentation
Enzyme-Linked Immunosorbent Assay - methods
Flow Injection Analysis - instrumentation
Flow Injection Analysis - methods
Reviews
Vitellogenins - analysis
Vitellogenins - metabolism
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - metabolism
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Summary:Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.
ISSN:0910-6340
1348-2246
1348-2246
DOI:10.2116/analsci.27.1069