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Sequential Injection Immunoassay for Environmental Measurements
Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because...
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Published in: | Analytical Sciences 2011/11/10, Vol.27(11), pp.1069-1069 |
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container_title | Analytical Sciences |
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creator | SOH, Nobuaki TANAKA, Mayumi HIRAKAWA, Koji ZHANG, RuiQi NAKAJIMA, Hizuru NAKANO, Koji IMATO, Toshihiko |
description | Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis. |
doi_str_mv | 10.2116/analsci.27.1069 |
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A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.</description><identifier>ISSN: 0910-6340</identifier><identifier>ISSN: 1348-2246</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.27.1069</identifier><identifier>PMID: 22076332</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Animals ; Biomarkers - analysis ; Biomarkers - metabolism ; Chemistry ; Environmental Monitoring - instrumentation ; Environmental Monitoring - methods ; Enzyme-Linked Immunosorbent Assay - instrumentation ; Enzyme-Linked Immunosorbent Assay - methods ; Flow Injection Analysis - instrumentation ; Flow Injection Analysis - methods ; Reviews ; Vitellogenins - analysis ; Vitellogenins - metabolism ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - metabolism</subject><ispartof>Analytical Sciences, 2011/11/10, Vol.27(11), pp.1069-1069</ispartof><rights>2011 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2011</rights><rights>Copyright Japan Science and Technology Agency 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c657t-a9cc379ad7d7f200d6b5e64724c96f3a70971c81583affebf836dc689b7471ab3</citedby><cites>FETCH-LOGICAL-c657t-a9cc379ad7d7f200d6b5e64724c96f3a70971c81583affebf836dc689b7471ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22076332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOH, Nobuaki</creatorcontrib><creatorcontrib>TANAKA, Mayumi</creatorcontrib><creatorcontrib>HIRAKAWA, Koji</creatorcontrib><creatorcontrib>ZHANG, RuiQi</creatorcontrib><creatorcontrib>NAKAJIMA, Hizuru</creatorcontrib><creatorcontrib>NAKANO, Koji</creatorcontrib><creatorcontrib>IMATO, Toshihiko</creatorcontrib><title>Sequential Injection Immunoassay for Environmental Measurements</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - metabolism</subject><subject>Chemistry</subject><subject>Environmental Monitoring - instrumentation</subject><subject>Environmental Monitoring - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - instrumentation</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Flow Injection Analysis - instrumentation</subject><subject>Flow Injection Analysis - methods</subject><subject>Reviews</subject><subject>Vitellogenins - analysis</subject><subject>Vitellogenins - metabolism</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>0910-6340</issn><issn>1348-2246</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp90U1L7DAUBuAgis5V1-5kwIV30_Hko_lYiYheBxQX6jqkaaod2lSTVph_f1NmHFTQTULIc96EcxA6wjAjGPMz400TbT0jYoaBqy00wZTJjBDGt9EEFIaMUwZ76E-MCwBMJCG7aI8QEJxSMkHnD-5tcL6vTTOd-4Wzfd356bxtB9-ZGM1yWnVheuXf69D5NsHk7pyJQ3DjKR6gnSp9wR2u9330dH31eHmT3d7_m19e3GaW56LPjLKWCmVKUYqKAJS8yB1ngjCreEWNACWwlTiX1FSVKypJeWm5VIVgApuC7qPTVe5r6NKHY6_bOlrXNMa7bohaAQPMAWiSf3-VGFKHCFf5SE--0UU3hLGlGjMmcykJhaTOVsqGLsbgKv0a6taEZYrS4xT0egqaCD1OIVUcr3OHonXlxn-0PQFYgZiu_LMLnx7-MfN6VbKIvXl2m0wT-to27ovH63Us3AD7YoJ2nv4HYiatGQ</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>SOH, Nobuaki</creator><creator>TANAKA, Mayumi</creator><creator>HIRAKAWA, Koji</creator><creator>ZHANG, RuiQi</creator><creator>NAKAJIMA, Hizuru</creator><creator>NAKANO, Koji</creator><creator>IMATO, Toshihiko</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7U1</scope><scope>7U2</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Sequential Injection Immunoassay for Environmental Measurements</title><author>SOH, Nobuaki ; 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subjects | Analytical Chemistry Animals Biomarkers - analysis Biomarkers - metabolism Chemistry Environmental Monitoring - instrumentation Environmental Monitoring - methods Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods Flow Injection Analysis - instrumentation Flow Injection Analysis - methods Reviews Vitellogenins - analysis Vitellogenins - metabolism Water Pollutants, Chemical - analysis Water Pollutants, Chemical - metabolism |
title | Sequential Injection Immunoassay for Environmental Measurements |
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