Synthesis and kinetic testing of new inhibitors for a metallo-β-lactamase from Klebsiella pneumonia and Pseudomonas aeruginosa

• Published in: European journal of medicinal chemistry 2011-12, Vol.46 (12), p.6075-6082
• Main Authors: Mohamed, Mosaad S., Hussein, Waleed M., McGeary, Ross P., Vella, Peter, Schenk, Gerhard, Abd El-hameed, Rania H.
• Format: Article
• Language: English
• Subjects: Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; beta-Lactamase Inhibitors; beta-Lactamases - metabolism; Biological and medical sciences; Enzyme Inhibitors - chemical synthesis; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Humans; Klebsiella Infections - drug therapy; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - enzymology; Medical sciences; Metallo-β-lactamase inhibitors; Pharmacology. Drug treatments; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - enzymology; Pseudomonas Infections - drug therapy; Pyrrole; Pyrroles - chemical synthesis; Pyrroles - chemistry; Pyrroles - pharmacology; Pyrrolo [2,3-d]pyrimidine; Structure–activity relationship
• ISSN: 0223-5234; 1768-3254
• DOI: 10.1016/j.ejmech.2011.10.030

There are currently no clinically useful inhibitors against metallo-β-lactamases (MBLs), enzymes that confer resistance against a broad spectrum of commonly used antibiotics and that are produced by an increasing number of bacterial pathogens. New pyrrole derivatives were synthesized and assayed for their inhibitory effect on the catalytic activity of the IMP-1 MBL from Pseudomonas aeruginosa and Klebsiella pneumoniae. Six compounds tested ( 3a-3c, 5, 7 and 8) show micromolar inhibition constants ( K i values range from ∼10 to 30 μM). In silico docking was employed to investigate the binding mode of the strongest inhibitor, 3b, in the active site of IMP-1. Implications for further improvements of binding efficiency and specificity are discussed. Surface view of the IMP-1 active site for the highest Autodock Vina score conformation of IMP-1 with 3b docked into the active site. [Display omitted] ► A new series of pyrrole derivatives was synthesized. ► Eleven pyrrole derivatives were tested as metallo-β-lactamase inhibitors. ► Six compounds showed a good inhibition effects with K i values ranging from ∼10 to 30 μM. ► In silico docking was employed to investigate the binding mode of the strongest inhibitor.