The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss

• Published in: The Journal of immunology (1950) 2011-01, Vol.186 (2), p.869-877
• Main Authors: Liang, Shuang, Krauss, Jennifer L, Domon, Hisanori, McIntosh, Megan L, Hosur, Kavita B, Qu, Hongchang, Li, Fenge, Tzekou, Apostolia, Lambris, John D, Hajishengallis, George
• Format: Article
• Language: English
• Subjects: Alveolar Bone Loss - immunology; Alveolar Bone Loss - metabolism; Alveolar Bone Loss - microbiology; Animals; Disease Models, Animal; Immune Evasion - genetics; Immune Evasion - immunology; Inflammation Mediators - metabolism; Inflammation Mediators - physiology; Interleukin-10 - physiology; Interleukin-12 - antagonists & inhibitors; Interleukin-12 - biosynthesis; Interleukin-12 - physiology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Myeloid Differentiation Factor 88 - physiology; Periodontitis - immunology; Periodontitis - metabolism; Periodontitis - microbiology; Porphyromonas gingivalis; Porphyromonas gingivalis - growth & development; Porphyromonas gingivalis - immunology; Receptor Cross-Talk - immunology; Receptor, Anaphylatoxin C5a - deficiency; Receptor, Anaphylatoxin C5a - metabolism; Receptor, Anaphylatoxin C5a - physiology; Signal Transduction - genetics; Signal Transduction - immunology; Toll-Like Receptor 2 - antagonists & inhibitors; Toll-Like Receptor 2 - physiology
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.1003252

The C5a anaphylatoxin receptor (C5aR; CD88) is activated as part of the complement cascade and exerts important inflammatory, antimicrobial, and regulatory functions, at least in part, via crosstalk with TLRs. However, the periodontal pathogen Porphyromonas gingivalis can control C5aR activation by generating C5a through its own C5 convertase-like enzymatic activity. In this paper, we show that P. gingivalis uses this mechanism to proactively and selectively inhibit TLR2-induced IL-12p70, whereas the same pathogen-instigated C5aR-TLR2 crosstalk upregulates other inflammatory and bone-resorptive cytokines (IL-1β, IL-6, and TNF-α). In vivo, the ability of P. gingivalis to manipulate TLR2 activation via the C5a-C5aR axis allowed it to escape IL-12p70-dependent immune clearance and to cause inflammatory bone loss in a murine model of experimental periodontitis. In the latter regard, C5aR-deficient or TLR2-deficient mice were both resistant to periodontal bone loss, in stark contrast with wild-type control mice, which is consistent with the interdependent interactions of C5aR and TLR2 in P. gingivalis immune evasion and induction of bone-resorptive cytokines. In conclusion, P. gingivalis targets C5aR to promote its adaptive fitness and cause periodontal disease. Given the current availability of safe and effective C5aR antagonists, pharmacological blockade of C5aR could act therapeutically in human periodontitis and reduce associated systemic risks.