Intranasally administered Lactobacillus gasseri TMC0356 protects mice from H1N1 influenza virus infection by stimulating respiratory immune responses

We conducted a study to evaluate the possibility that intranasal administration of a new probiotic strain Lactobacillus gasseri TMC0356 (TMC0356) may protect host animals from influenza virus (IFV) infection, which was indicated by enhanced respiratory immune responses in a mouse model. After 3 days...

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Published in:World journal of microbiology & biotechnology 2011-02, Vol.27 (2), p.411-416
Main Authors: HARATA, Gaku, FANG HE, HIRUTA, Naoyuki, KAWASE, Manabu, KUBOTA, Akira, HIRAMATSU, Masaru, YAUSI, Hisako
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biotechnology
Cytokines
Cytotoxicity
Disease prevention
Fundamental and applied biological sciences. Psychology
Infections
Influenza
Influenza virus
Laboratories
Lactobacillus gasseri
Lungs
Morbidity
Mortality
Probiotics
Studies
Survival
Swine flu
Tumor necrosis factor-TNF
Viral infections
Viruses
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Summary:We conducted a study to evaluate the possibility that intranasal administration of a new probiotic strain Lactobacillus gasseri TMC0356 (TMC0356) may protect host animals from influenza virus (IFV) infection, which was indicated by enhanced respiratory immune responses in a mouse model. After 3 days of exposure to TMC0356, BALB/c mice were intranasally infected with IFVA/PR/8/34 (H1N1). Lung cells were isolated from the tested mice and evaluated for cytotoxicity against YAC-1 cells. After intranasal treatment with TMC0356, mice showed a lower morbidity and higher survival rate compared to control mice (P < 0.05). The cytotoxicity of lung cells isolated from mice after intranasal treatment against YAC-1 cells was statistically higher than that of lung cells isolated from control mice (P < 0.05). Intranasal administration of TMC0356 significantly increased mRNA expression of interleukin (IL)-1β, tumor necrosis factor, IL-10, and monocyte chemotactic protein-1 (P < 0.01). These results suggest that intranasal administration of TMC0356 may protect the host animal from IFV infection. They also indicate that TMC0356 can enhance respiratory cell-mediated immune responses of host animals characteristically with up-regulated activation of lung natural killer cells. Further studies will evaluate the possible role of the immune stimulatory effects of TMC0356 within the protective effects of this bacterium against IFV, as observed in the present study. [PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-010-0472-x