MALAT-1 enhances cell motility of lung adenocarcinoma cells by influencing the expression of motility-related genes

MALAT-1, a long non-coding RNA, is associated with metastasis, but its role in the metastatic process remains unknown. Here, we show that short-interfering RNA-mediated MALAT-1 silencing impaired in vitro cell motility of lung cancer cells and influenced the expression of numerous genes. In these ge...

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Bibliographic Details
Published in:FEBS letters 2010-11, Vol.584 (22), p.4575-4580
Main Authors: Tano, Keiko, Mizuno, Rie, Okada, Tomoko, Rakwal, Randeep, Shibato, Junko, Masuo, Yoshinori, Ijiri, Kenichi, Akimitsu, Nobuyoshi
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Adenocarcinoma of Lung
Cell Line, Tumor
Cell motility
Cell Movement - genetics
Gene Expression Regulation, Neoplastic - genetics
Humans
Lung adenocarcinoma
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Metastasis
Non-coding RNA
RNA Interference
RNA Processing, Post-Transcriptional
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - genetics
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
Transcription, Genetic
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Summary:MALAT-1, a long non-coding RNA, is associated with metastasis, but its role in the metastatic process remains unknown. Here, we show that short-interfering RNA-mediated MALAT-1 silencing impaired in vitro cell motility of lung cancer cells and influenced the expression of numerous genes. In these genes, knockdown of any one of CTHRC1, CCT4, HMMR, or ROD1 clearly inhibited cell migration. In MALAT-1 knockdown cells, pre-mRNA levels were decreased in some but not all genes. Thus, our findings suggest that MALAT-1 is a novel class of non-coding RNA that promotes cell motility through transcriptional and post-transcriptional regulation of motility related gene expression.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.10.008