DNA demethylation and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra Arn. cell culture

Embryogenic cell lines initiated from immature zygotic embryos of Pinus nigra Arn. ssp. Austriaca were characterized in terms of macromorphological traits (colour, bipolar structures formation, germination ability) and their embryogenic potential was defined as high, medium or null. Quantification o...

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Bibliographic Details
Published in:Trees (Berlin, West) West), 2009-12, Vol.23 (6), p.1285-1293
Main Authors: Noceda, Carlos, Salaj, Terézia, Pérez, Marta, Viejo, Marcos, Cañal, Maria Jesús, Salaj, Ján, Rodriguez, Roberto
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
DNA methylation
Embryos
Forestry
Life Sciences
Original Paper
Pine trees
Pinus nigra
Plant Anatomy/Development
Plant Pathology
Plant Physiology
Plant Sciences
Polyamines
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Summary:Embryogenic cell lines initiated from immature zygotic embryos of Pinus nigra Arn. ssp. Austriaca were characterized in terms of macromorphological traits (colour, bipolar structures formation, germination ability) and their embryogenic potential was defined as high, medium or null. Quantification of global genomic DNA methylation revealed the existence of specific DNA methylation levels for the determinated embryogenic potentials. The line considered as effectively embryogenic, i.e., with the ability of develop the whole embryogenic program and producing plants, showed the lowest methylation levels. There was also proved the existence of an inverse relationship between total contents of free PAs and embryogenic potential, being the highest contents of free putrescine and spermidine in the non-embryogenic line and the lowest in the effectively embryogenic one. Relationships among DNA methylation levels, profiles of free individual polyamine contents and embryogenic potentials based on the ability to produce well-formed somatic embryos with effective plant conversion are discussed.
ISSN:0931-1890
1432-2285
DOI:10.1007/s00468-009-0370-8