RAPD analysis of genome variability of planted ginseng,Panax ginseng

Genome variability of 23 ginseng plants (Panax ginseng) grown in culture in Primorskii Krai was studied by RAPD method. Eleven arbitrary chosen primers were used to analyze 138 loci of DNA samples, 17 of which appeared to be polymorphic. The OPD-11-1000 fragment was found to be a RAPD marker allowin...

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Bibliographic Details
Published in:Molecular biology (New York) 2000-03, Vol.34 (2), p.297-302
Main Authors: Artyukova, E V, Kozyrenko, M M, Reunova, G D, Muzarok, T I, Zhuravlev, Yu N
Format: Article
Language:English
Subjects:
Gene polymorphism
Genetics
Genomes
Ginseng
Panax ginseng
Random amplified polymorphic DNA
Reintroduction
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Summary:Genome variability of 23 ginseng plants (Panax ginseng) grown in culture in Primorskii Krai was studied by RAPD method. Eleven arbitrary chosen primers were used to analyze 138 loci of DNA samples, 17 of which appeared to be polymorphic. The OPD-11-1000 fragment was found to be a RAPD marker allowing plants to be differentiated according to their morphotype. Using five primers, it was demonstrated that the genetic polymorphism of the cultivated plants is lower than that in nature (7.6% and 10.6%, respectively). Dendrograms of genetic relatedness are in accord with genetic differences between individuals of plantedP. ginseng belonging to different morphotypes, and demonstrate close relatedness of one of the morphotypes to wild plants. This morphotype could be recommended for reintroduction into natural habitats.
ISSN:0026-8933
1608-3245
DOI:10.1007/BF02759655