Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange

Transgenic Washington navel orange [Citrus sinensis (L.) Osbeck] plants were obtained using Agrobacterium-mediated transformation of seedling epicotyl tissue. An average of 45% (58 out of 128 segments) of the epicotyl segments produced shoots expressing the beta-glucuronidase (GUS)-intron reporter g...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell reports 1998-12, Vol.18 (3/4), p.229-234
Main Authors: Bond, J.E, Roose, M.L
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium tumefaciens
Agronomy. Soil science and plant productions
beta-glucuronidase
Biological and medical sciences
Biotechnology
Citrus aurantiifolia
Citrus limon
Citrus paradisi
Citrus reticulata
Citrus sinensis
Cultivars
culture media
enzyme activity
epicotyls
Fruits
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
Genetic engineering
Genetic engineering applications
genetic improvement
Genetic technics
genetic transformation
Genetics and breeding of economic plants
in vitro culture
methodology
Methods. Procedures. Technologies
micropropagation
neomycin
phosphotransferases (phosphomutases)
Plant breeding: fundamental aspects and methodology
Poncirus trifoliata
reporter genes
Seedlings
Shoots
Transgenic animals and transgenic plants
Transgenic plants
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transgenic Washington navel orange [Citrus sinensis (L.) Osbeck] plants were obtained using Agrobacterium-mediated transformation of seedling epicotyl tissue. An average of 45% (58 out of 128 segments) of the epicotyl segments produced shoots expressing the beta-glucuronidase (GUS)-intron reporter gene when using Agrobacterium strain C58 C1, compared to 29% (38 out of 128 segments) for EHA101-5 and 0% for LBA4404. Co-culture of 21-day-old Washington navel epicotyl stem segments gave greater transformation efficiency than co-culture of 35- or 56-day-old stem segments. After 6 weeks, regenerated shoots were micro-grafted in vivo onto seedling rootstocks of Carrizo citrange. Stable integration of the transgene sequence was confirmed by expression of the plant intron-containing GUS gene, PCR and Southern hybridization. The apomictic (non-zygotic) state of the transgenic plants was confirmed by isoenzyme and random amplified polymorphic DNA analyses. More than 50 transgenic plants have been obtained and are growing in the greenhouse.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050562