Evaluation of an immunochromatographic dip-strip test for the detection of Cryptosporidium oocysts in stool specimens

The study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting Cryptosporidium oocysts in stool samples. A total of 75 samples were tested, including 50 positive for Cryptosporidium spp. by acid-fast stain, 20 positive for other paras...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2002-08, Vol.21 (8), p.624-625
Main Authors: LLORENTE, M. T, CLAVEL, A, VAREA, M, OLIVERA, S, CASTILLO, F. J, SAHAGUN, J, RUBIO, M. C, GOMEZ-LUS, R
Format: Article
Language:English
Subjects:
Animals
Ascaris
Biological and medical sciences
Blastocystis hominis
Cryptosporidiosis
Cryptosporidiosis - diagnosis
Cryptosporidiosis - immunology
Cryptosporidium
Cryptosporidium meleagridis
Cryptosporidium parvum
Cryptosporidium parvum - immunology
Cryptosporidium parvum - isolation & purification
Endolimax nana
Entamoeba coli
Feces - microbiology
Genotypes
Giardia lamblia
Human protozoal diseases
Humans
Immunologic Tests - methods
Immunologic Tests - standards
Infectious diseases
Medical sciences
Oocysts
Oocysts - immunology
Oocysts - isolation & purification
Parasites
Parasitic diseases
Protozoal diseases
Sensitivity and Specificity
Strongyloides stercoralis
Trichuris trichiura
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Summary:The study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting Cryptosporidium oocysts in stool samples. A total of 75 samples were tested, including 50 positive for Cryptosporidium spp. by acid-fast stain, 20 positive for other parasites ( Blastocystis hominis, Endolimax nana, Entamoeba coli, Giardia lamblia, Ascaris lumbricoides, Strongyloides stercoralis and Trichuris trichiura), and five negative samples. The observed sensitivity was 98%, while specificity was 100%; the detection threshold was near 1000 oocysts/ml. Correctly diagnosed positive samples included Cryptosporidium parvum genotypes 1 and 2, whereas the single false-negative sample corresponded to a Cryptosporidium meleagridis infection.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-002-0778-1