Effect of the US3 protein of bovine herpesvirus 5 on the actin cytoskeleton and apoptosis

The US3 protein is a unique protein kinase only present in the Alphaherpesvirinae subfamily of the herpesviruses. Studies performed with several alphaherpesviruses demonstrated that the US3 protein is involved in cytoskeleton modifications during viral infection and displays anti-apoptotic activity....

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Bibliographic Details
Published in:Veterinary microbiology 2011-12, Vol.153 (3), p.361-366
Main Authors: Ladelfa, María Fátima, Kotsias, Fiorella, Del Médico Zajac, María Paula, Van den Broeke, Céline, Favoreel, Herman, Romera, Sonia Alejandra, Calamante, Gabriela
Format: Article
Language:English
Subjects:
Actin cytoskeleton
Actins - metabolism
Alphaherpesvirinae
Animals
Apoptosis
Apoptosis - physiology
Biological and medical sciences
Bovine herpesvirus 5
Cattle
Cell Nucleus - enzymology
Cercopithecus aethiops
Cytoplasm - enzymology
Cytoskeleton - metabolism
Fundamental and applied biological sciences. Psychology
Herpesvirus 5, Bovine - enzymology
Microbiology
Microtubules - metabolism
Miscellaneous
Molecular Sequence Data
Mutation
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
US3 kinase-dead
Vero Cells
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
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Summary:The US3 protein is a unique protein kinase only present in the Alphaherpesvirinae subfamily of the herpesviruses. Studies performed with several alphaherpesviruses demonstrated that the US3 protein is involved in cytoskeleton modifications during viral infection and displays anti-apoptotic activity. However, the US3 protein of BoHV-5 has not been studied up to now. As reported for other alphaherpesviruses, our results showed that BoHV-5 US3 confers resistance against apoptosis and induces cytoskeletal reorganization leading to cell rounding, actin stress fiber breakdown and cell projections that interconnect cells. The expression of a kinase-dead version of BoHV-5 US3 showed that the anti-apoptotic activity and the induction of cell projections are kinase-dependent whereas kinase activity is not absolutely required for actin stress fiber breakdown. Besides, the kinase-dead version of US3, but not the wild type protein, was found excluded from the nucleus. These results constitute the first report on the BoHV-5 US3 functions, and highlight that there are functional differences and similarities among US3 proteins of different alphaherpesviruses.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2011.05.037