Diversity distributions of killer cell immunoglobulin-like receptor genes and their ligands in the Chinese Shaanxi Han population

Abstract In the present study, 17 killer cell immunoglobulin-like receptors (KIR) genes and KIR ligands (human leukocyte antigen [HLA] –A and -B) were detected by using a polymerase chain reaction–sequence-specific primer (PCR-SSP) method in 104 unrelated healthy Han individuals living in Shaanxi pr...

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Published in:Human immunology 2011-09, Vol.72 (9), p.733-740
Main Authors: Wang, Hong-dan, Zhu, Bo-feng, Shen, Chun-mei, Fan, Ai-ying, Song, Tie-niu, Liu, Jia-li, Qin, Hai-xia, Deng, Li-bin, Fan, Shuan-liang, Huang, Qi-zhao, Guo, You-feng, Fang, Jie, Zhang, Ping, Yan, Chun-xia
Format: Article
Language:English
Subjects:
Allergy and Immunology
China
Chinese Shaanxi Han population
Ethnic Groups
Gene Frequency
Genetics, Population
Genotyping Techniques
Haplotypes - genetics
HLA-A Antigens - genetics
Humans
KIR
PCR-SSP
Phylogeny
Polymorphism, Genetic
Receptors, KIR - genetics
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Summary:Abstract In the present study, 17 killer cell immunoglobulin-like receptors (KIR) genes and KIR ligands (human leukocyte antigen [HLA] –A and -B) were detected by using a polymerase chain reaction–sequence-specific primer (PCR-SSP) method in 104 unrelated healthy Han individuals living in Shaanxi province, China. The observed carrier frequencies of the 12 KIR genes ranged from 0.14 to 0.96. KIR2DL4, 3DL2, 3DL3, 2DP1 and 3DP1 were found to be present in every individual. A total of 51 different KIR gene profiles were identified, in which 11 gene profiles exclusively belonged to the study population. Neighbor-joining phylogenetic tree between the studing population and its neighboring ethnic groups was constructed using the observed carrier frequencies of 13 KIR loci. The phylogenetic tree shows that the Shaanxi Han population, Han populations in different regions, Yi, Japanese, and Koreans were in the same cluster. KIR/HLA relationships show that KIR3DS1− /3DL1+ /Bw4+ was the most common association in the population. In conclusion, the present study findings reveal the high polymorphism of KIRs in the Shaanxi Han population, demonstrate the KIR/HLA association in the study population, and enrich the KIR and HLA gene resources. The obtained KIR data will further the understanding of genetic relationships among populations in different geographic areas, and assist in answering questions regarding KIR/HLA relationships.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2011.04.003