Qualitative and quantitative analysis of serum immunoglobulins of four Antarctic fish species

Immunoglobulins from the Antarctic fish species Trematomus bernacchii, Trematomus hansoni, Trematomus newnesi, and Chionodraco hamatus were analysed in whole serum and after purification by affinity chromatography on protein A-sepharose. Using SDS-PAGE, the apparent masses of the heavy and light cha...

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Bibliographic Details
Published in:Polar biology 1997-09, Vol.18 (3), p.209-213
Main Authors: SCAPIGLIATI, G, CHAUSSON, F, COOPER, E. L, SCALIA, D, MAZZINI, M
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Antibody production
Biological and medical sciences
Chionodraco hamatus
Dicentrarchus labrax
Enzymes
Fish
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Heterogeneity
Immunobiology
Immunoglobulins
Marine
Marine biology
Trematomus bernacchii
Trematomus hansoni
Trematomus newnesi
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Summary:Immunoglobulins from the Antarctic fish species Trematomus bernacchii, Trematomus hansoni, Trematomus newnesi, and Chionodraco hamatus were analysed in whole serum and after purification by affinity chromatography on protein A-sepharose. Using SDS-PAGE, the apparent masses of the heavy and light chains were, respectively, 83.5kDa and 27.5kDa for T. bernacchii, 83.5 kDa and 27 kDa for T. hansoni, 81 kDa and 27.5 kDa for T. newnesi, and 74.5 kDa and 30 kDa for C. hamatus. It was not possible to purify immunoglobulins from T. newnesi due to their low concentration in serum. Heterogeneity in mass of both heavy and light chains was observed in all species. By using a polyclonal antibody raised against sea bass immunoglobulins, cross-reactivity was observed with heavy and light chains of all species. With this antibody, an indirect enzyme-linked immunosorbent assay (ELISA) was developed and results showed the relative immunoglobulin concentration in sera of the Antarctic fish species considered, using as standard sea bass immunoglobulins.[PUBLICATION ABSTRACT]
ISSN:0722-4060
1432-2056
DOI:10.1007/s003000050177