Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts

• Published in: Anaerobe 2011-06, Vol.17 (3), p.97-105
• Main Authors: Ripamonti, Barbara, Agazzi, Alessandro, Bersani, Carla, De Dea, Paola, Pecorini, Chiara, Pirani, Silvia, Rebucci, Raffaella, Savoini, Giovanni, Stella, Simone, Stenico, Alberta, Tirloni, Erica, Domeneghini, Cinzia
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; anti-infective agents; Antibiosis; Antimicrobial agents; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus coagulans; Bacterial Adhesion; Bacterial Typing Techniques; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Bifidobacterium longum; Calves; calving; Cattle - microbiology; Cell Line; Cluster Analysis; Fatty Acids, Volatile - biosynthesis; Feces; Feces - microbiology; functional properties; gastrointestinal system; Gastrointestinal tract; Humans; Intestines - microbiology; Lactic Acid - biosynthesis; Lactic acid bacteria; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus animalis; Lactobacillus paracasei; Lactobacillus paracasei subsp. paracasei; Microbial Sensitivity Tests; Microbial Viability; nucleotide sequences; Probiotic characterization; probiotics; Probiotics - chemistry; Probiotics - isolation & purification; rearing; ribosomal RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; screening; Species Specificity; Species-specific lactic acid bacteria; Streptococcus; Streptococcus bovis; Streptococcus macedonicus; Veal; veal calves
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2011.05.001

The selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. The aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, Bifidobacterium longum and Bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. For this purpose, bacterial strains isolated from faeces collected from 40 healthy 50-day-calves, were identified by RiboPrinter and 16s rRNA gene sequence. The most frequent strains belonged to the species B. longum, Streptococcus bovis, Lactobacillus animalis and Streptococcus macedonicus. Among these, 7 strains were chosen for testing their probiotic characteristics in vitro. Three strains, namely L. animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and B. coagulans SB117 showed varying individual but promising capabilities to survive in the gastrointestinal tract, to adhere, to produce antimicrobial compounds. These three selected species-specific bacteria demonstrated in vitro, both singularly and mixed, the functional properties needed for their use as potential probiotics in veal calves.