Ancylobacter abiegnus sp. nov., an oligotrophic member of the xylotrophic mycobacterial community

• Published in: Microbiology (New York) 2010-08, Vol.79 (4), p.483-490
• Main Authors: Zaichikova, M. V, Berestovskaya, Yu. Yu, Akimov, V. N, Kizilova, A. K, Vasilieva, L. V
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology; Mycobacterium
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261710040107

A new species of the genus Ancylobacter, exemplified by strain Z-0056, was isolated from dystrophic humified waters formed by xylotrophic fungi grown on decaying spruce wood. The cells of strain Z-0056 (0.65-0.9 μm) are coccoid, gram-negative, with fimbriae, and nonmotile. The strain is pleomorphic and reproduces by nonuniform division. Strain Z-0056 is an aerobic organoheterotroph and a mesophilic and moderately acidophilic oligotrophic microorganism. As an inhabitant of dystrophic ultrafresh waters, strain Z-0056 is sensitive to NaCl. The bacterium utilizes organic acids (acetate, pyruvate, oxalate, gluconate, malate, succinate, and citrate), as well as xylose and xylan. The microorganism grows in a pH range of 4.0-8.0, with an optimum at pH 5.5. The temperature range for growth is 15-25°C, with an optimum at 20°C. According to its ecophysiological properties, strain Z-0056 belongs to the group of ombrophilic dissipotrophs. The DNA G+C base content is 66.8 mol %. According to phylogenetic analysis, strain Z-0056 belongs to the genus Ancylobacter. Strain Z-0056 showed the highest similarity (98.3%) with the type strain of the species A. oerskovii. The phenotypic and phylogenetic properties of strain Z-0056 support classification of this microorganism within the genus Ancylobacter as the novel species Ancylobacter abiegnus sp. nov.