Biosynthesis of silver and gold nanoparticles using thermophilic bacterium Geobacillus stearothermophilus

Nanomaterials have assumed a great deal of importance as they often display unique and considerably modified physical, chemical and biological properties as compared to their counterparts of the macroscale. In this study, biogenic synthesis of silver and gold nanoparticles by Geobacillus stearotherm...

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Bibliographic Details
Published in:Process biochemistry (1991) 2011-10, Vol.46 (10), p.1958-1962
Main Authors: Mohammed Fayaz, A., Girilal, M., Rahman, Mashihur, Venkatesan, R., Kalaichelvan, P.T.
Format: Article
Language:English
Subjects:
absorption
biosynthesis
Capping proteins
Fourier transform infrared spectroscopy
Geobacillus stearothermophilus
Gold
Gold nanoparticles
Maxima
Monodispersed
nanogold
Nanoparticles
nanosilver
polyacrylamide gel electrophoresis
Polydispersed
Proteins
salts
secretion
Secretions
Silver
Silver nanoparticles
Stearothermophilus
thermophilic bacteria
Transmission electron microscopy
X-ray diffraction
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Summary:Nanomaterials have assumed a great deal of importance as they often display unique and considerably modified physical, chemical and biological properties as compared to their counterparts of the macroscale. In this study, biogenic synthesis of silver and gold nanoparticles by Geobacillus stearothermophilus has been attempted. The exposure of G. stearothermophilus cell free extract to the metal salts leads to the formation of stable silver and gold nanoparticles in the solution. These nanoparticles were characterized by UV–Vis spectra, FTIR, TEM, and XRD. The silver and gold nanoparticles have absorption maxima at 423nm and 522nm respectively. The TEM micrograph revealed the formation of polydispersed particles in the case of silver nanoparticles and monodispersed particles with respect to the gold nanoparticles. High stability of the nanoparticle solution could be attributed to the secretion of certain capping proteins by the bacterium in the reaction mixture. The involvement of these proteins was confirmed by FTIR and SDS PAGE.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2011.07.003