In Situ Forming and Rutin-Releasing Chitosan Hydrogels As Injectable Dressings for Dermal Wound Healing

An in situ gel-forming system composed of rutin- and tyramine-conjugated chitosan derivatives, horseradish peroxidase (HRP), and hydrogen peroxide (H2O2) was prepared and applied to dermal wound repair. Rutin was employed to enhance production and accumulation of extracellular matrix in the healing...

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Bibliographic Details
Published in:Biomacromolecules 2011-08, Vol.12 (8), p.2872-2880
Main Authors: Tran, Ngoc Quyen, Joung, Yoon Ki, Lih, Eugene, Park, Ki Dong
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Bandages
Biological and medical sciences
Cell Line
Chitosan - chemistry
Exact sciences and technology
General pharmacology
Hydrogels
In Vitro Techniques
Magnetic Resonance Spectroscopy
Male
Medical sciences
Mice
Natural polymers
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Physicochemistry of polymers
Rats
Rats, Sprague-Dawley
Rutin - administration & dosage
Skin - physiopathology
Starch and polysaccharides
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Wound Healing
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Summary:An in situ gel-forming system composed of rutin- and tyramine-conjugated chitosan derivatives, horseradish peroxidase (HRP), and hydrogen peroxide (H2O2) was prepared and applied to dermal wound repair. Rutin was employed to enhance production and accumulation of extracellular matrix in the healing process. In vitro study demonstrates that released rutin significantly enhanced cell proliferation as compared with media without rutin. In vivo wound healing study was performed by injecting hydrogels on rat dorsal wounds with a diameter of 8 mm for 14 days. Histological results demonstrated that rutin-conjugated hydrogel exhibited enhancement of wound healing as compared with treatments with PBS, hydrogel without rutin, and a commercialized wound dressing (Duoderm). More specifically, rutin-conjugated hydrogels induced better defined formation of neo-epithelium and thicker granulation, which is closer to the original epithelial tissue. As a result, this study suggests that the in situ gel-forming system can be a promising injectable gel-type wound dressing.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm200326g