Time-kill studies with micafungin and voriconazole against Candida glabrata intracellularly in human monocyte-derived macrophages and extracellularly in broth

Abstract The purpose of this study was to determine by time-kill methodology the anticandidal effectiveness and durability of micafungin (MCF) and voriconazole (VRC), singly and in combination, against Candida glabrata ( Cgl ), intracellularly in human monocyte-derived macrophages and extracellularl...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2011-08, Vol.70 (4), p.468-474
Main Authors: Baltch, Aldona L, Ritz, William, Bopp, Lawrence H, Michelsen, Phyllis B, Smith, Raymond P
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Antifungal combinations
Biological and medical sciences
Candida glabrata
Candida glabrata - drug effects
Drug Synergism
Echinocandins - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Lipopeptides - pharmacology
Macrophages - microbiology
Medical sciences
Micafungin
Microbial Sensitivity Tests
Microbial Viability - drug effects
Microbiology
Miscellaneous
Mycology
Pyrimidines - pharmacology
Time Factors
Time-kill methodology
Triazoles - pharmacology
Voriconazole
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Summary:Abstract The purpose of this study was to determine by time-kill methodology the anticandidal effectiveness and durability of micafungin (MCF) and voriconazole (VRC), singly and in combination, against Candida glabrata ( Cgl ), intracellularly in human monocyte-derived macrophages and extracellularly in RPMI-MOPS broth with and without fetal calf serum (FCS) or pooled human serum (PHS). The anticandidal activity of MCF was concentration-dependent and durable. Combinations of MCF + VRC both intra- and extracellularly were more effective than single drugs. However, in extracellular experiments, FCS, and even more PHS, significantly decreased the anticandidal activity of MCF and VRC. Our in vitro studies suggest that MCF alone may be adequate where protein concentration is low (intracellular or extravascular sites), while MCF + VRC combinations may be preferred where protein concentration is high such as in the intravascular space.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2011.04.008